Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB091

Poster Communications

Antioxidant Effects of Apocynin on Ovarian Ischemia-Reperfusion Induced Remote Lung Injury in Rats

A. Tanyeli2, D. Guzel1, S. Doganay1, S. Comakli4, E. Polat3

1. Faculty of Medicine, Physiology, Sakarya Universty, Sakarya, Turkey. 2. Faculty of Medicine, Physiology, Atatürk University, Erzurum, Turkey. 3. Faculty of Medicine, Biochemistry, Atatürk University, Erzurum, Turkey. 4. Veterinary Faculty, Pathology, Atatürk University, Erzurum, Turkey.

Interruption of blood flow stimulates the release of molecules that cause accumulation of blood cells, including polymorphonuclear leukocytes (PMN) in the lungs. Cell activation and releasing molecules can lead to acute respiratory failure. Due to the endogenous antioxidant system is inadequate because of massive reactive oxygen radicals, the exogenous molecules may be helpful in reducing oxidative damage. Inhibition of this pathway by Apocynin (Apo) has been shown to reduce oxygen radicals. We aimed to investigate the remote tissue effects of Apo in ovarian I/R injury. Wistar Albino female rats (n=40) were divided into five groups: Sham operation (Group I), ischemia/reperfusion (Group II), ischemia/reperfusion plus dimethyl sulfoxide (Group III), ischemia/reperfusion plus apocynin 20 mg/kg (Group IV) and ischemia/reperfusion plus apocynin 50 mg/kg (Group V). Bilateral adnexal 3-h period of ischemia was performed, followed by 3-h of reperfusion in study groups. Lung tissue MDA, TAS, TOS, SOD and MPO were measured using spectrophotometric methods. TOS, OSI, MDA and MPO levels were significantly higher, whereas TAS, SOD levels were lower in Group II than in Group I. Similar statistical results were determined in Group I and Group III comparisons. In treatment groups, when compared to Group II, TOS, OSI, SOD and MPO values were significantly changed. Our results suggested that, apocynin, an NADPH inhibitor, reduced I/R-induced inflammation and regulated the oxidative balance.

Where applicable, experiments conform with Society ethical requirements