Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB092

Poster Communications

The Effects of Apocynin on Inflammatory Cytokines And Apoptosis in Ischemia-Reperfusion Induced Remote Lung Injury

A. Tanyeli2, D. Guzel1, S. Doganay1, S. Comakli3, E. Polat4

1. Physiology, Sakarya University, Faculty of Medicine, Sakarya, Turkey. 2. Physiology, Atatürk University, Faculty of Medicine, Erzurum, Turkey. 3. Pathology, Atatürk University, Veterinary Faculty, Erzurum, Turkey. 4. Biochemistry, Atatürk University, Faculty of Medicine, Erzurum, Turkey.

Under physiological conditions, in the organism, there is a protective balance between antioxidants and oxidants against harmful effects of free radicals. In ischemic reperfusion damage via the metabolic pathway named "respiratory burst" catalyzed by NOX during phagocytosis, ROS production may increase excessively, and it cannot be balanced against the antioxidant defense system, which leads to the tissue damage. Apocynin is one of the best-known inhibitors of the NOX family. We evaluated the effects of apocynin on inflammatory cytokines, cell death and apoptosis in ischemia-reperfusion (IR) induced remote lung injury. Wistar Albino female rats (n=40) were divided into five groups: Sham operation (Group I), IR (Group II), IR plus dimethyl sulfoxide (Group III), IR plus apocynin 20 mg/kg (Group IV) and IR plus apocynin 50 mg/kg (Group V). Bilateral adnexal 3-h period of ischemia was performed, followed by 3-h of reperfusion in study groups. TNF-α, IL-1β, NF-κB, Caspase-3 and LC3B were measured using immunohistopathological methods. Vascular congestion and hemorrhage were higher in Group II and group III. Apocynin treatment decreased the congestion and hemorrhage. Immunohistochemical staining of NF-κB, IL-1β, TNF-α, Caspase-3, and LC3B immunopositivities were observed at the highest values in group II and group III, whereas in the treatment groups, immunopositivities gradually decreased in inverse proportion to the dose of the apocynin. Ischemia reperfusion injury, which increases tissue apoptosis, cell death and inflammatory cytokine content, plays an important role in the pathogenesis of remote organ damage. Apocynin administration plays a promising protective role in remote lung injury.

Where applicable, experiments conform with Society ethical requirements