Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB094

Poster Communications

Investigation of Cytotoxic Properties of Hetero-Ring Substituted Organophosphazene Compounds

S. Sandal1, S. Tekin1, K. Koran2, A. Gorgulu2

1. Department of Physiology, Inonu University Medical School, Malatya, Turkey. 2. Department of Chemistry, Firat University, Faculty of Science, Elazig, Turkey.

  • Figure 1. General structure of the compounds.

Phosphazene compounds in the group of phosphorus-nitrile compounds are also called phosphonitrile and hydroazophosphorine. Phosphazene compounds have a cyclic or linear structure in which two organic or inorganic side groups (R) are attached to each phosphorus atom, consisting of repeated -P = N-units in the structures. These compounds constitute a class of flavonoids and are abundant in edible plants. This study was designed to determine the posible cytotoxic effects of new hetero-ring substituted organophosphazene compounds on viability of two different types of human cancer cells. New hetero-ring full substituted organophosphazene analogues (FSC 1,2) have been synthesized from the reaction of 3-(2,3-dihidrokbenzofuran-5-il)-1-(4-hidroksifenil)prop-2-en-1-one (HAF-1), and 1-(4-Hidroksifenil)-3-(6-metoksipiridin-3-il)prop-2-en-1-one (HAF-2) with hexachlorocyclotriphosphazene (HCCP), respectively (Figure 1). The structure characterizations of all cyclotriphosphazenes were confirmed by FT-IR and NMR spectroscopy methods (31P, 1H and 13C-APT). The human epithelial ovarian cancer cell line (A2780) and the human prostate cancer cell line (PC-3) were treated with different concentrations of the compounds (1, 5, 25, 50 and 100 μM) [1,3]. In vitro cytotoxic properties of FSC 1,2 were determined using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay method. The LogIC50 values of FSC 1,2 were determined by using a Graphpad prism 6 programs. The high concentrations of the tested compounds caused significantly reduces in the cell viability both A2780 and PC3 cell lines (p<0.05). Our results indicated that hetero-ring substituted organophosphazene compounds can be candidate molecule for cancer treatment. However, there is need for comprehensive studies to reveal the effect of the substance.

Where applicable, experiments conform with Society ethical requirements