Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB096

Poster Communications

The effect of Chitosan Nanosilver Dressing Versus Mesenchymal Stem Cells on Wound Healing

S. Ghannam1, H. Korayem1, L. Farghaly1, S. Hosny1

1. Histology, Genetics & Cell Biology, Suez Canal University, Ismailia, Egypt.

Wounds constitute a major worldwide public health problem. Infection is the most common complication of wounds. Surgical wound infection is still a major post operative problem. New modalities for treating wound infections such as nanotechnology and stem cell therapy have been tried. However, the available studies didn't compare the effect of mesenchymal stem cells against Chitosan nanosilver on wound-healing and didn't use histological techniques in assessing their effects. This study compares the effect of chitosan nanosilver dressing with the intradermal injection of bone marrow mesenchymal stem cells on wound healing. Forty adult female albino rats were equally randomized into 4 groups: group I served the control, in group II, two surgical wounds of 4 mm diameter were induced on the back of each animal. In group III chitosan nanosilver dressing was stitched over the wounds and group IV received intradermal injection of 1x 106mesenchymal stem cells derived from bone marrow after wound induction. All animals were scarified after seven days by decapitation. Two skin specimens with 3 mm of the surrounding skin were obtained from the two wounds of each animal and processed for histological, (Haematoxylin and Eosin stain, Masson's trichrome stain, periodic acid-Schiff reaction) immunohistochemical (Proliferating cell nuclear antigen-PCNA) and immunofluorescent (mesenchymal stem cells labeling) techniques. Wound group showed complete shedding of epidermis, decreased mean optical density of collagen fibers, negative PAS reaction and negative PCNA immunostaining. Chitosan nanosilver dressing group showed significant increase in the mean epidermal thickness (147.2 ± 21.2) , collagen optical density (0.258 ± 0.08) , PAS optical density (0.195 ± 0.024) and the positive PCNA immunoreactivity (0.673 ± 0.111) compared to the mesenchymal stem cells group parameters (Mean epidermal thickness:135 ± 24.3, collagen optical density: 0.146 ± 0.03, PAS optical density: 0.183±0.022 & PCNA immunoreactivity: 0.572 ± 0.104). In conclusion, both chitosan nanosilver dressing and bone marrow mesenchymal stem cells promoted wound healing, but the non-invasive chitosan nanosilver dressing had better and faster wound healing effect.

Where applicable, experiments conform with Society ethical requirements