Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB105

Poster Communications

NADPH Oxidase Inhibitor Apocynin Ameliorates Renal Ischemia-Reperfusion Injury by Regulation of Inflamation, Autophagy and Apoptosis

D. Guzel1, A. Tanyeli2, S. Doganay1, S. Comakli4, E. Polat3

1. Faculty of Medicine, Physiology, Sakarya Universty, Sakarya, Turkey. 2. Faculty of Medicine, Physiology, Atatürk University, Erzurum, Turkey. 3. Faculty of Medicine, Biochemistry, Atatürk University, Erzurum, Turkey. 4. Veterinary Faculty, Pathology, Atatürk University, Erzurum, Turkey.

The present study aimed to investigate the antioxidant, antiinflammatory and antiapoptotic effects of NADPH Oxidase Inhibitor apocynin on the rat ovary against ischemia-reperfusion injury using histopathological methods. Forty Wistar-albino female rats were subjected to Sham operation (S), ischemia/reperfusion (IR), ischemia/reperfusion plus dimethyl sulfoxide (IR-D), IR plus apocynin 20 mg/kg (APO20), and IR plus apocynin 50 mg/kg (APO50). In all IR groups, a bilateral adnexal 3-h period of ischemia was performed, followed by 3-h of reperfusion. A single dose of apocynin was given intraperitoneally 15 min before ischemia and just before reperfusion in APO20 and APO50 groups. TNF-α, IL-1β, NF-κB, Caspase-3 and LC3B were measured using histopathological methods. Immunohistochemical staining of NF-κB, IL-1β, TNF-α, Caspase-3, and LC3B immunopositivities were observed in the IR groups at the highest values, whereas in the treatment groups, immunopositivities gradually decreased in inverse proportion to the dose of the apocynin. In the ovary tissue hemorrhage and congestion were found to be intense in the IR groups, whereas in the treatment groups, hemorrhage and congestion were alleviated in inverse proportion to apocynin dose. In conclusion, apocynin decreases IR-induced inflamation, apoptosis and autophagic cell death in a dose-dependent manner. Thus, apocynin may be a new alternative for the treatment of diseases with increased cell death, where oxidant stress is intense.

Where applicable, experiments conform with Society ethical requirements