Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB130

Poster Communications

Protective Effect of NADPH Oxidase Inhibitor Apocynin on Ischemia-Reperfusion Injury In The Ovarian Tissue

D. Guzel1, A. Tanyeli2, S. Doganay1, S. Comakli3, E. Polat4

1. Faculty of Medicine, Physiology, Sakarya Universty, Sakarya, Turkey. 2. Faculty of Medicine, Physiology, Atatürk University, Erzurum, Turkey. 3. Veterinary Faculty, Pathology, Atatürk University, Erzurum, United Kingdom. 4. Faculty of Medicine, Biochemistry, Atatürk University, Erzurum, Turkey.

Ovarian ischemic injury, although seen rarely, is one of the important gynecological emergency disorders, especially as a result of ovary torsion. Following ischemia, oxygen deficiency and free radical accumulation occur in the ovaries. For reestablished tissue viability, reperfusion is required, although abundant blood causes more toxic products and results in reperfusion injury. Apocynin (APO) is one anti-inflammatory, antioxidant agent that acts as a potent and a selective inhibitor of NADPH oxidase (NOX) . To evaluate the role of APO in ischemia/reperfusion-induced ovarian injury, forty Wistar Albino female rats were subjected to Sham operation (S), ischemia/reperfusion (IR), IR plus dimethyl sulfoxide (IR-D), IR plus apocynin 20 mg/kg (APO20), and IR plus apocynin 50 mg/kg (APO50). In all IR groups, a bilateral adnexal 3-h period of ischemia was performed, followed by 3-h of reperfusion. A single dose of apocynin was given intraperitoneally 15 min before ischemia and just before reperfusion in APO20 and APO50 groups. MDA, TAS, TOS, SOD, and MPO levels were measured using biochemical methods. In IR, MPO, MDA, TOS and OSI increased in significant level, while TAS and SOD decreased. Significant difference in TAS and OSI were seen in APO20. APO50 caused significant induction in TAS and SOD and reduction in OSI and MDA levels in comparison to the IR. Our findings suggested that the apocynin, a NADPH inhibitor, reduces oxidative damage induced by IR in ovarian tissue and restores the oxidative balance.

Where applicable, experiments conform with Society ethical requirements