Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB149

Poster Communications

Role of p-Coumaric acid in alleviating of the intestinal Ischemia/Reperfusion injury

A. Tanyeli1, F. Ekinci Akdemir2, E. Eraslan3, M. C. Guler1, S. Ozbek Sebin1, I. Gulcin4

1. Department of Physiology, Faculty of Medicine, Ataturk University, Erzurum, Turkey. 2. Department of Nutrition and Dietetics, High School of Health, Agri Ibrahim Cecen University, Agri, Turkey. 3. Department of Physiology, Faculty of Medicine, Bozok University, Yozgat, Turkey. 4. Department of Biochemistry, Faculty of Science, Ataturk University, Erzurum, Turkey.

Intestinal Ischemia/Reperfusion (I/R) injury is an undesirable consequence of a multitude pathologies such as small bowel transplantation, cardiopulmonary bypass, hernias, neonatal necrotizing enterocolitis and abdominal aortic aneurysm surgery. Various therapy procedures have been conducted to reduce or eliminate intestinal I/R injury in experimental models to date. This study was planned to investigate the protective role of p-coumaric acid against intestinal I/R injury. For this purpose, an experimental intestinal I/R model was established in our study. In the p-coumaric acid and I/R groups, superior mesenteric artery was clamped for 1 h. Then, the clamp was removed and reperfusion was allowed for 2 h. The beginning of bleeding of the tissue and the pinking of the color were accepted as reperfusion. At the end of 2h, rats were sacrificed and intestinal tissue samples were collected rapidly. It was determined that MPO activity, MDA levels, TOS and OSI values increased due to severe oxidative damage depending on I/R. In addition, TAC value and SOD activity decreased in the I/R group. TAC value and SOD activity increased while TOS, OSI values, MPO activity and MDA level were reducing in the I/R + p-coumaric acid (50 and 100 mg/kg bw.) groups. In the p-coumaric acid 100 mg/kg group, TAC value and SOD activity more increased and OSI, TOS values more decreased compared with p-coumaric acid 50 mg/kg group. Different doses of the p-coumaric acid demonstrated protective effects against intestinal I/R (1h: 2h) injury in experimental animals

Where applicable, experiments conform with Society ethical requirements