Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB195

Poster Communications

Hesperidin attenuates oxidative ovarian damage injured by ischemia-reperfusion

F. Ekinci Akdemir1, A. Tanyeli2, D. Guzel3

1. Department of Nutrition and Dietetics, Ağri Ibrahim Cecen University, High School of Health, Ağri, Turkey. 2. Physiology, Atatürk University, Faculty of Medicine, Erzurum, Turkey. 3. Physiology, Sakarya University, Faculty of Medicine, Sakarya, Turkey.


The purpose of this research is to examine the possible protective effects of Hesperidin as antioxidants on ovary injured by the ischemia reperfusion. We used 24 Wistar-type female rats within the scope of our study. The rats were randomly divided into 3 groups. Our groups are planned as follows; sham-operated group (only laparotomy was performed without ischemia reperfusion), ischemia reperfusion group (laparotomy was performed and the ovaries were exposed to torsion 360 degrees for 3 hours followed by detorsion for 3 hours for reperfusion but no medication was given), ischemia reperfusion+hesperidin group (first, ischemia was performed, then hesperidin 100 mg/kg i.p was administered and finally reperfusion was performed). Using the enzyme-linked immunosorbent assay kits, total antioxidant level (TAS), superoxide dismutase (SOD) were measured as antioxidant; total oxidant level (TOS), myeloperoxidase (MPO) and malondialdehyde (MDA) levels were measured as oxidant parameters in ovarian tissue. One way ANOVA and Tukey test were performed. When our results of biochemical analysis were evaluated, it was determined that the oxidant parameters increased in the ischemia-reperfusion group but the antioxidant activity decreased significantly (p<0.05). Malondialdehyde level and total oxidant status value were decreased in the group treated with hesperidin, but superoxide-dismutase activity was found to be increased. These results have shown us that single dose administration of hesperidin is highly effective against oxidative ovarian damage caused by ischemia reperfusion.

Where applicable, experiments conform with Society ethical requirements