Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB200

Poster Communications

Down expression of onco-suppressive CHEK-2, HOXB-13 genes and Up-regulation of Proto-onco AR gene in prostate cancer

M. J. Aslam1, M. Faisal1, H. Muzaffar1, A. Mahmood1

1. Institute of pharmacy, Physiology and Pharmacology, Faisalabad, Punjab, Pakistan.

Cancer is group of interconnected diseases in which cells of the body starts growing and their growth go uncontrolled. It is 2nd main cause of death around the world after cardiovascular diseases being on number 1. Prostate gland is the main male accessory gland involved in sperm growth and lubrication. Among all the cancers, prostate cancer is the 2nd most diagnosed cancer in men across the globe. Molecular and genetic players of prostate cancer have been studied extensively in modern research era. Role of some genes such as BRCA1, BRCA2, PTEN, HOXB13, AR and CHECK2 is evident as tumor suppressor in progression of prostate cancer. Objective of this study was to observe the down expression of HOXB13, CHEK2 genes and Up-regulation of AR gene in male prostate carcinogenesis and understand the back stage signaling pathways involved in prostate cell aberration. Biopsy samples of prostate gland of affected patients was collected locally from PINUM health care facility with permission of ethical review committee and samples were kept in solution containing 0.9% normal saline and 10% formalin. After qRT-PCR analysis, it was observed that CHEK2 and HOXB13 genes were down expressed while Ar gene over expressed in patients samples which in turn favor the causation of cancer. The variance of data was analyzed through ANOVA test and DMR test was used to observe significance of data.

Where applicable, experiments conform with Society ethical requirements