Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB202

Poster Communications

Investigation of the Effect of Nifedipine on Kidney Catalase of Ischemia/Reperfusion Injured Rat

E. Eraslan1, A. Tanyeli2, E. Senturk3, M. Güler2, M. Sentürk4

1. Physiology, Bozok University, Faculty of Medicine, Yozgat, Turkey. 2. Physiology, Atatürk University, Faculty of Medicine, Erzurum, Turkey. 3. Physiology, Ağri Ibrahim Cecen University, High School of Health, Ağri, Turkey. 4. Biochemistry, Agri Ibrahim Cecen University, Pharmacy Faculty, Ağri, Turkey.

AIM: Catalase (CAT) is a common enzyme found in nearly all organisms exposed to oxygen it catalyzes the decomposition of hydrogen peroxide to water and oxygen. The purpose of this study is to investigate the effect of nifedipine drug on rat kidney tissue CAT enzyme in ischemia / reperfusion (I / R) and I / R injured rats. METHODS: Control, sham, I / R (1:24 hours) and nifedipine + I / R are as follows: A total of 24 male Wistar albino rats weighing 250-300 g were equally and randomly divided into four groups. In the last group, nifedipine was administered at the 4 mg/kg dose before intraperitoneal ischemia period. Kidney tissues were removed after bilateral I/R process. Tissue levels of enzyme activity of CAT were measured. CAT activity was determined by Aebi method which H2O2 at 240 nm. RESULTS: Specific activity values were determined for CAT enzyme at four different experimental groups. Groups were determined as follows: Control group 0.652 EU/mg protein, sham group 0.668 EU/mg protein, I/R group 1.027 EU/mg protein, and nifedipin + I/R group 1.714 EU/mg protein. CONCLUSION: As a result, the activity of the CAT enzyme was determined to be mostly activated in nifedipine + I/R group among all applications. This demonstrates that the secretion is further increased in each administration compared to the control.

Where applicable, experiments conform with Society ethical requirements