Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB203

Poster Communications

Ghrelin's effects on Levels of Metalloproteinases and Inflammatory parameters in septic intestinal tissue

G. Ates Ulucay1,2, E. Ozkok3, H. Yorulmaz4, V. Olgac5, S. Tamer2

1. Department of Physiology, Istanbul Yeni Yuzyil University, Faculty of Medicine, Istanbul, Turkey. 2. Department of Physiology, Istanbul University, Istanbul Medical Faculty, Istanbul, Turkey. 3. Department of Neuroscience, Istanbul University, Aziz Sancar Institute of Experimental Medicine, Istanbul, Turkey. 4. Department of Physiology, Halic University, Istanbul, Turkey. 5. Department of Pathology, Istanbul University, Istanbul, Turkey.

Motivation/problem statement: Sepsis is a multisystemic organ dysfunction, lead to tissue injury and organ failure by excessive activation of complement components and neutrophil actions against to bacterial infection (1). Lipopolysaccharide has been reported to induce cytokine release and production of reactive oxygen species, leading to inflammation, oxidative stress and tissue damage (2). Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) are biomarkers that include prognosis of sepsis (3). MMP-9 and TIMP-1 groups were associated with higher mortality in the onset of severe sepsis. YKL-40 is a 40 kd glycoprotein as known glycoprotein 39, chitinase 3Y like protein 1, involving in remodelling of extracellular matrix (4). YKL-40 probably participates in inflammation and remodeling/degradation of the extracellular matrix/damaged tissue. Ghrelin is known that an endogen ligand of growth hormone and it shows anti-inflammatory and anti-oxidant effect (5). In this study,we aimed to investigate the effects of exogenous Ghrelin on YKL-40, MMP-2, MMP-9, TIMP-1 immunoreactivities in the small intestine tissue in LPS induced septic rats. Methods/procedure/approach: The approvals were obtained from Istanbul University Animal Experiments Local Ethics Committee for our study. Wistar albino male rats (180-230g, for each of group n=6) were divided into 4 groups: control, LPS, Ghrelin, LPS + Ghrelin. LPS were given twice dose first, 5 mg / kg i.v. and second dose 5 mg / kg i.p. Ghrelin 10 nmol / kg i.v. was administered in a single dose. Twenty-four hours after the first injections, the rats were decapitated. Intestinal tissue and blood samples were taken. MMP-2, MMP-9, YKL-40, and TIMP-1 immunoreactivities were detected by immunohistochemical staining with using proper antibodies on small intestinal tissue sections. Results: In the histological examination; it was found higher immunoreactivities of TNF-a, MMP-2, MMP-9, and YKL-40 in the LPS group. However, IL-10 immunoreactivity was found lower in the LPS group. Conclusion/implications: We may proposed that Ghrelin is improved on metalloproteinases and inflammatory parameters in damaged small intestinal tissue in LPS induced septic rats. Ghrelin decreased proinflamatory cytokine level and protective on elevated matrix metalloproteinases and YKL-40 levels in damaged small intestinal tissue in LPS induced septic rats.

Where applicable, experiments conform with Society ethical requirements