Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB207

Poster Communications

Beneficial effect of oat β-glucans on the inflammation-related gene expression in colitis

E. Zyla1, K. Dziendzikowska1, D. Kamola2, J. Wilczak2, M. Oczkowski1, T. Krolikowski1, M. Gajewska2, J. Harasym3, J. Gromadzka-Ostrowska1

1. Department of Dietetics, Faculty of Human Nutrition and Consumer Sciences, Warsaw University of Life Sciences, Warsaw, Poland, Warsaw, Poland. 2. Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland. 3. Institute of Food Chemistry and Technology, Wroclaw University of Economics, Wroclaw, Poland.

β-glucans are polysaccharides belonging to the soluble fiber fraction, extractable from e.g. cereals and classified as a physiologically active. The positive impact of β-glucans on organisms result from their immune-stimulating properties and ability to binding to immune cells receptors, immune cells activation, and regulation of humoral and cell-mediated immunity. The aim of the study was to determine the effects of the dietary supplementation with chemically pure high- and low-molecular-weight oat β-glucans on the gene expression of inflammatory cytokines and receptors in rats colon tissue with colitis. Adult male Spraque Dawley rats (380±20g, n=54) were divided into two groups: control (n=27) and with experimentally-induced colitis (n=27, single administration of 2,4,6-trinitrobenzenosulfinic acid (TNBS), per rectum). In the control groups 0.9% NaCl was administered in the same way. Both experimental and control groups were divided into three subgroups fed during 21-day AIN-93M feed supplemented with 3% (w/w) of (1) low molecular weight β-glucan (βGl+, n=9), (2) high molecular weight β-glucan (βGh+, n=9) or (3) feed without β-glucan (βG-, n=9). At the end of the feeding period under Isoflurane anesthesia colon samples were collected. RNA was then isolated and reverse transcribed into cDNA (Qiagen). Targeted RT-qPCR (RT2 Profiler PCR Array Rat Inflammatory Cytokines & Receptors, Qiagen) was performed to analyze inflammatory-related gene expression. Data analysis was performed using the online Qiagen analysis software (RT2 profiler PCR array data analysis V3.5) with >2 fold-change and p<0.05 considered to be statistically significant. Result indicate that out of 84 genes TNBS (in colitis (βG-) group) compared to control (βG-) induced up-regulation of 32 genes (including chemokines and its receptor genes: Ccl3, Ccl4, Cll12, Ccl17, Ccl19, Ccl22, Ccr3, Ccr4, Ccr5, Ccr6, Ccr8, Cxcr1, Cxcr2, Cxcr3, Cxcr5; interleukin and its receptor genes: Il10ra, Il13, Il16, Il1rn, Il21, Il2rb, Il2rg, Il5ra, Il6r and other genes involved in inflammation: Faslg, Lta, Ltb, Osm, Spp1, Tnf, Tnfsf11, Tnfsf14). The addition of β-glucan to animal feed significantly altered gene expression level in the rats with colitis (in colitis (βGl+) up-regulation of: Cxcl1, Il17a, Cxcr1, Spp1 and down-regulation of: Ccl19, Cd40lg, Cxcr5, Il10ra, Il16, Il21, Il2rg, Il5ra, Lta, Ltb, Osm, Tnf, Tnfsf11, Tnfsf14) (in colitis (βGh+) up-regulation of: Cxcl1, Cxcl2, Cxcr2, Il17a, Il1a, Spp1 and down-regulation of: Ccl19, Cd40lg, Cxcr5, Lta, Ltb, Tnfsf11) with stronger protective effect demonstrated by low molecular weight β-glucan. The obtained results indicate the protective effect of β-glucan on the colon, which in consequence may reduce the negative effects of inflammation in the large intestine.

Where applicable, experiments conform with Society ethical requirements