Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB211

Poster Communications

Exposure to kerosene fumes deteriorates reproductive functions in male Sprague Dawley rats.

I. I. Olatunji-Bello1, O. M. Olumide1, F. K. Olaribigbe1, A. A. Sanni1

1. Department of Physiology, Lagos State University College of Medicine, Ikeja, Ikeja, Nigeria.

Daily exposure to crude oil and its products as air pollutants can affect the body functions. The implications of exposure to kerosene fumes on reproductive parameters and oxidative stress markers were therefore investigated in male rats. Male albino rats (n=20) weighing 150-200 g were distributed into 2 groups of control (exposed to normal atmospheric air) and kerosene (KERO) (exposed to kerosene fumes) daily for 3hours in the exposure chamber for a period of 3 weeks. Serum level of testosterone, Luteinizing hormone (LH) and follicle stimulating hormone (FSH) were assayed using Enzyme-linked Immuno Assay (ELISA) kit. Epididymal sperm count, motility and morphology were assessed, activities of antioxidant enzymes; catalase (CAT), glutathione reductase (GSH), superoxide dismutase (SOD) and lipid peroxidase; malondialdehyde (MDA) in the serum and testis were measured. Testicular histopathology was also investigated. Rats were sedate with chloroform and sacrificed following cervical dislocation, values are represented as ±SEM and compared with ANOVA Results from the study showed a lower (p<0.05) sperm count (58.00±2.08 x106sp/ml vs 71.00±2.12 x106 sp/ml), motility (85.75±3.57% vs 98.50±0.96%) and higher number of abnormal sperm cells (13.00±2.31% vs 11.33±1.67%) in KERO when compared with control. Serum level of testosterone (1.25±0.21 mg/ml vs 2.40±0.29 mg/ml) was significantly lower in the KERO group while the reduced serum level of LH and FSH in KERO was not significant when compared with compared. The CAT (19.56±0.66 mg/ml vs 23.73±0.45 mg/ml) and GSH (49.31±3.16 µmol/ml vs 62.86±2.13 µmol/ml) activities in the serum were also significantly lower while MDA (6.20±0.27 nmol/ml vs 4.26±0.27 nmol/ml) was significantly higher in the KERO than in control. In the testis, GSH (22.85±3.15 µmol/ml vs 35.13±3.49 µmol/ml) was lower (p<0.05) while MDA (6.35±0.86 nmol/ml vs 3.55±0.64 nmol/ml) was higher (p<0.05) in the KERO than in control. Histology of the testis showed alterations of the sperm cells and reduction of interstitial cells in the KERO when compared with the control. In conclusion, frequent inhalation of kerosene fumes produces oxidative stress which deteriorates male reproductive functions in albino rats.

Where applicable, experiments conform with Society ethical requirements