Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB215

Poster Communications

Association of serum Leptin level with body mass index and lipid profile in Diacetylmorphine (Heroine) addicts versus healthy controls.

S. H. Habib1, S. H. Khizar2, M. Malik1

1. Physiology, Institute of Basic Medical Sciences, Khyber Medical University Pakistan, Peshawar, KPK, Pakistan. 2. Physiology, KMU Institute of Medical Sciences, Kohat, Pakistan.

Background: Diacetylmorphine (DAM) addiction commonly known as heroin addiction, is a common socioeconomic problem markedly affecting the nutritional and metabolic status of the individuals. The circulating adipose tissue derived hormone Leptin is an important marker in regulating body weight and lipid metabolism. Previous reports suggest derangement of Leptin in DAM addicts. In this study our study was to compare circulating concentration leptin and its relationship to body mass index and lipid profile in DAM addicts in comparison to normal healthy individuals. Methodology: This was a cross sectional analytical study including 41 male DAM addicts and 41 male healthy controls between 25 to 46 years of age. Detail history and physical examination along with baseline laboratory tests for exclusion of infection were performed before recruiting the subjects for participation in the study. Standard method was used to calculate the anthropometric measures. Blood samples were corrected to calculate serum lipid profile, complete blood picture. Serum leptin was calculated using enzyme linked immunosorbent assay (ELISA) technique. All statistical analysis was done using SPSS version 22. Numerical data was presented as mean and standard deviation. Independent sample T test was applied to compare mean of the two groups. Ethical approval was obtained from the ethical review committee of Khyber Medical University, Peshawar, Pakistan. Results: Basal serum leptin levels in DAM addicts were significantly decreased compared to controls (3.08 ± 0.13 pg/ml vs 3.24 ± 0.24 pg/ml; p<0.001). Furthermore, LDL and hemoglobin were also significantly reduced in the addicts compared to controls (71.8 ± 25.99 vs 105.39 ± 39.09; p<0.001 and 13.44 ± 0.78 vs 14.73 ± 0.91; p<0.001) respectively. The difference between other parameters were no significant. Univariate and Multivariate regression did not find any significant association of Leptin with BMI and lipid profile in DAM addicts (p=0.198), however, significant association of Leptin with BMI was observed in normal subjects (p=0.027). Conclusion: In conclusion, circulating concentrations of leptin are decreased in heroin addiction. Moreover, positive correlation of serum leptin levels with body mass index and lipid profile is lost in addicts in contrast to control group.

Where applicable, experiments conform with Society ethical requirements