Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB224

Poster Communications

Study of Uterine Contractions in Pregnant Diabetic Rats: Role of Oxidative Stress
Faten M. A. Diab, Aziza S.El-Naser, Mona A. Ahmed, Doaa M. Abdel Wahed, Manal S Abd-El hamid and Faiza A. El-Hamdy*

F. M. Ali1, A. S. El- Naser1, M. A. Ahmed1, D. M. AbdelWahed1, M. S. Abd-El hamid1, F. A. El-Hamdy1,2

1. Medical Physiology, Faculty of Medicine, Ain Shams University, Cairo, Egypt. 2. Physiology, Medical Faculty, Benghazi, Libya.

Background: Uterine contractility plays a major role in human reproduction. Dysfunctional uterine contractility, especially met with gestational diabetes mellitus (GDM), increases perinatal morbidity and mortality. GDM impairs the function of most smooth muscle containing organs, as the uterus; and the oxidative stress mechanism underlying organ damage has been implicated. Objective: This work was conducted to study the changes imposed on rat's myometrium contractility, and the effect of vitamin E as a supplement with insulin treatment in modulating the diabetic-induced complications that develop in experimental gestational diabetes. Materials & Methods: 80 time-dated pregnant Albino rats were studied. GDM was induced by intra-peritoneal injection of 40 mg/kg streptozotocin at gestational day 5. Rats were allocated into five groups: control pregnant (group 1), untreated diabetic (group 2), insulin-treated diabetic (group 3) vitamin E-treated diabetic (group 4) and combined insulin & vitamin-E treated (group 5). On gestation day 18, all rats were subjected for recording of spontaneous basal uterine muscle activity in isolated organ bath, and its responses to the uterotonic agent; oxytocin and to the tocolytic agent; atosiban. The plasma glucose, insulin and HbA1c levels, together with serum lipid profile; total cholesterol, triglycerides, HDL-c, LDL-c were assayed. The HOMA-IR and atherogenic index were calculated. Besides, serum malondialdehyde and total antioxidant capacity levels were estimated. Results: Untreated diabetic (group 2) established significant reduction in uterine motility, with insulin resistance, dyslipidemia, enhanced oxidative stress state. Following insulin treatment alone, (group 3) demonstrated significant decrease in HbA1c, persistence of insulin resistance, with relative improvement in lipid profile and uterine motility, with still of altered oxidative stress status. With vitamin E treatment alone, (group 4) showed significant improvement of uterine motility, and amelioration of oxidative stress state but still altered hyperglycaemia and dyslipidemia. The best results were observed in the combined treatment (group5), where significant improvement in uterine motility, together with full correction to the glycaemic, lipidemic and oxidative stress states. Conclusion: Vitamin E, as a supplement with insulin treatment in gestational diabetes, offers beneficial role in improving the contractile machinery state of the myometrium via restoring the normal redox balance. Key Words: Gestational diabetes mellitus- Myometrium contractility - Vitamin-E. *MD thesis, Physiology Department, Faculty of Medicine, Ain Shams University.

Where applicable, experiments conform with Society ethical requirements