Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB227

Poster Communications

Effect of Vitamin C on glucose metabolism in partial and total sleep-deprived male Wistar rat

T. Kpai2, S. T. Shittu1, O. J. Ogundele1, T. J. Lasisi1

1. Physiology, University of Ibadan, Port Harcourt, Rivers, Nigeria. 2. Human Kinetics, Health and Safety Education, Ignatius Ajuru University of Education, Port Harcourt, Rivers, Nigeria.


Chronic sleep deprivation is associated with elevations in oxidative stress and impaired glucose homeostasis. Vitamin C (a well-known antioxidant) prevented sleep-deprivated induced memory impairment, however it is not known if Vitamin C has any effect on impaired glucose metabolism during sleep deprivation. This study investigated the effect of vitamin C administration on glucose homeostasis in 7 days sleep deprived animals. Thirty male Wistar rats weighing 150-200 gram were divided into six (6) groups as Control; control + Vitamin C; partial sleep deprived; partial sleep deprived + Vitamin C; total sleep deprived; and total sleep deprived + Vitamin C. The animals were sleep deprived for 7 days using the modified multiple platform chambers. Partial sleep deprivation was achieved by sleep depriving the animals for 20 hours daily and allowed to rest for the remaining 4 hours while total sleep deprivation is when the animals were not allowed the daily 4 hours rest. Groups 2,4 and 6 were daily administered Vitamin C (300mg/kg) by oral gavage. At the end of the experiment, the animals were anaesthetized using ketamine (100mg/kg body weight, i.p). Blood samples were obtained for fasting blood glucose level and lactate determination. Muscle and liver samples were obtained for glycogen determination while hexokinase and glucose-6-phosphatase activities were determined in the liver only. Differences in means were compared using student's t-test and ANOVA (Analysis of Variance) and p value was set as p < 0.05. There was significant (p < 0.05) decrease in body weight of the total sleep deprived rats compared to the control, the rate of the decrease body weight was however minimized in the total sleep deprived that were administered vitamin C. The fasting blood glucose level and plasma lactate were significantly reduced in both the total sleep deprived and total sleep deprived + Vitamin C groups compared to the control. Muscle glycogen was significantly depleted in total sleep deprived and the total sleep deprived + Vitamin C groups however, hepatic glycogen was increased in the total sleep deprived animals but decreased significantly in the total sleep deprived + Vitamin C animals. Glucose 6-phosphatase activity was significantly decreased in the partial sleep deprived animals while treatment with Vitamin C had no significant effect in the partial sleep deprived + Vitamin C group; it was however, increased in the total sleep deprived group but decreased significantly in the total sleep deprived + Vitamin C group. Hexokinase activity was decreased in the total sleep deprived animals but increased in the total sleep deprived + Vitamin C. It was concluded that partial sleep deprivation had no effect on glucose metabolism but total sleep deprivation caused derailment in glucose metabolism which was not reversed by treatment with vitamin C.

Where applicable, experiments conform with Society ethical requirements