Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB228

Poster Communications

Anti-oxidant supplementation ameliorates noradrenaline and serotonin contents in perimenopausal female rats following acute restraint stress.

O. A. Akinnibosun1, A. P. Arikawe1, A. W. Olusanya2, I. C. Udenze3, A. M. Aghoghovwia1, T. E. Adedeji1, K. F. Bajomo1, A. W. Erem1

1. Physiology, College of Medicine, University of Lagos, Lagos, Lagos, Nigeria. 2. Pharmacology, Therapeutics and Toxicology, College of Medicine, University of Lagos, Lagos, Nigeria. 3. Clinical Pathology, College of Medicine, University of Lagos, Lagos, Nigeria.


The decreased antioxidant defense characteristic of perimenopause leads to a decrease in stress resilience upon exposure to external stressors. Female 28-day old Sprague-Dawley rats were divided into 3 groups; Control group (injected s.c., Corn oil 2.5 μl/g BW); VCD group (injected s.c., 4-vinylcyclohexene diepoxide 160 mg/kg BW diluted in Corn oil) both for 15 days; and Aged group allowed to age naturally till 150 days. Seven weeks after VCD/corn oil administration, and 150 days in Aged group, rats were further divided into 3 sub-groups: L-Arginine (oral, 100mg/kg BW), D-ribose (oral, 200mg/kg BW) and a third group received neither for additional 30 days. At 130 days in Control and VCD groups, and 180 days in Aged group on diestrus morning, animals were subjected to acute restraint stress for 30 minutes. Blood samples were drawn before and after stress from the retro-orbital sinus for measurement of serum noradrenaline and serotonin concentrations using HPLC technique. Ovaries were processed through HPLC for assessment of noradrenaline and serotonin contents. Values are means ± S.E.M., compared by ANOVA. In unstressed and stressed animals, D-ribose significantly decreased (p < 0.05) serum noradrenaline and serotonin in Aged (Noradrenaline: 44.90±3.54µg/ml, 34.85±9.45µg/ml respectively; Serotonin: 3.49±0.26µg/ml, 4.43±0.97µg/ml respectively) and VCD (Noradrenaline: 29.05±2.24µg/ml, 13.13±3.94µg/ml respectively; Serotonin: 4.35±0.33µg/ml, 3.78±0.55µg/ml respectively) groups compared to Control group (Noradrenaline: 63.66±3.46µg/ml, 59.95±5.45µg/ml respectively; Serotonin: 6.73±0.51µg/ml, 6.64±0.62µg/ml respectively). It also significantly increased (p < 0.05) ovarian noradrenaline and serotonin of stressed animals in Aged (5.34±0.23µg/g, 24.58±0.13µg/g respectively) and VCD (4.12±0.10µg/g, 14.54±2.10µg/g respectively) groups compared to Control group (2.43±0.14µg/g, 8.51±2.04µg/g respectively). L-Arginine significantly decreased (p < .l0.05) serum serotonin of unstressed and stressed animals in Aged (4.13±0.42µg/ml, 3.30±0.30µg/ml respectively) and VCD (3.13±0.18µg/ml, 2.49±0.16µg/ml respectively) groups compared to Control group (6.70±0.13µg/ml, 5.30 ± 0.28µg/ml respectively). In both unstressed and stressed animals, L-Arginine significantly increased (p < 0.05) ovarian noradrenaline and serotonin in Aged (Noradrenaline: 6.38±0.94 µg/g, 2.01±0.15µg/g respectively; Serotonin: 12.78±0.92µg/g, 15.56±0.65µg/g respectively) and VCD (Noradrenaline: 6.63±0.95µg/g, 2.22±0.36µg/g respectively; Serotonin: 14.14±0.98µg/g, 6.26±0.57µg/g respectively) groups compared to Control group (Noradrenaline: 3.25±0.31µg/g, 1.11±0.26µg/g respectively; Serotonin: 8.31±0.94µg/g, 2.22±0.38µg/g respectively). These results show that L-Arginine and D-Ribose supplementation helped to prevent an exacerbation of stress hormonal response.

Where applicable, experiments conform with Society ethical requirements