Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB229

Poster Communications

Ocimum gratissimum Leaf Extract May Precipitate Infertility in Male Diabetic Wistar Rats

S. T. Shittu1, S. Shittu1, A. Olatunji1, W. Oyeyemi2

1. Physiology, University of Ibadan, Ibadan, OYO, Nigeria. 2. Physiology, Igbinedion University, Okada, Edo, Nigeria.


This study was designed to investigate the effect of Ocimum gratissimum (OG) on sperm quality and testicular cytoarchitecture in alloxan-induced diabetic rats. Twenty male Wistar rats (150-200 g) were assigned into 4 groups (n=5) as A (control), B (OG), C (Dia) and D (Dia+OG). Groups A and B were normal animals administered distilled water or OG (400 mg/kg), respectively while diabetes was induced by alloxan monohydrate (100 mg/kg) in groups C and D followed by administration of distilled water or OG, respectively for 28 days. Blood samples were obtained for fasting blood glucose (FBG) and fructosamine determination while, epididymis and testes were obtained for sperm quality assessment using computer-assisted sperm analyser and testicular histomorphometry, respectively. Seminiferous tubule diameter and interstitial space distance were quantified in hematoxylin and eosin stained slides. Statistical analysis was done using ANOVA and student t test at α0.05. Fructosamine and FBG were reduced in Dia+OG (80.11±3.80 μmol/L and 132.0±8.41 mg/dl, respectively) compared with Dia (139.66±4.29 μmol/L and 285.6±26.69 mg/dl, respectively). Sperm count was unchanged in Dia but decreased in OG and Dia+OG; abnormal sperm cells increased in OG, Dia and Dia+OG. Mild vacuolation in the seminiferous tubule, disorganized germinal cells layer, arrested sperm maturation with empty spermatozoa in lumen, decreased seminiferous tubule diameter and increased interstitial space were observed in the testes of OG, Dia and Dia+OG compared with control. it was concluded that diabetes mellitus induces sperm impairments and distortions in testicular cytoarchitecture which was aggravated by OG leaf extract in male Wistar rats.

Where applicable, experiments conform with Society ethical requirements