Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB236

Poster Communications

Effects of noopept on glucose, insulin, insulin resistance and pancreas of streptozotocin-induced diabetic prepubertal rats

P. GÜRBÜZ1, H. DUZOVA1, C. C. GÜL2, A. C. TASLIDERE2

1. Physiology, Inonu University, Medical School, Malatya, Turkey. 2. Embryology and Histology, Inonu University, Inonu University, Malatya, Turkey.


The main purpose of type 1 diabetes mellitus (T1DM) management is blood glucose control. Since insulin resistance is higher than normal in puberty, problems arise in regulating insulin dosage in treatment. Usage of higher doses of insulin in treatment causes insulin resistance to rise and this leads higher daily insulin requirements. Because of this, new approaches to T1DM treatment are needed. Noopept is a synthetic nootropic dipeptide that is used as a cognitive regulator. Studies with noopept have suggested it to have anti-diabetic properties. In one study noopept has been shown to have incretin effect in glucose regulation, by inhibiting β cell degeneration. In our study, we tried to determine effects of noopept on; blood glucose-insulin levels, insulin resistance, and pancreas in prepubertal DM rats. In the study 60 prepubertal, 28 day-old, male, Sprague Dawley rats were divided into 6 groups (n=10/group). i) control, ii) DM control, iii) noopept, iv) DM+noopept, v) DM+insulin, vi) DM+insulin+noopept. Diabetes model was formed by 50 mg/kg streptozotosin on postnatal 28th day. At the end of the third day after streptozotosin administration, rats with blood glucose levels above 200 mg/dl were considered to have diabetes. Intraperitoneally 0.5 mg/kg noopept and 1 unit insulin was administered for 14 days in required groups. Blood glucose levels were measured at days 0, 3, 7 and 14 of the experiment. The research ended on the 46th day. Insulin levels were determined from sera by ELISA test and insulin resistance was determined by HOMA-IR formula. Pancreatic tissues were also histologically evaluated. After diabetes formation, blood glucose levels were found to be low in the noopept treated groups (p <0.05). Insulin levels of DM control group were significantly higher than DM+ Noopept+insulin group (p = 0.05). Insulin resistance of DM control group was higher than all other groups. DM + Noopept+Insulin group's insulin resistance was lower than DM+Insulin group (p <0.05). When pancreas was evaluated histologically, DM control group was found to have cellular distribution loss, vacuolization, vascular congestion, cellular infiltration. Islet cells of DM control group had dense small nuclei, vacuolated cytoplasm. Also edemas in vessels, degeneration of Langerhans islets were observed in DM control group. These changes were observed less frequently in the treated groups. Modifications were minimal in DM+Noopept+Insulin group. At the end of the research we conclude that, noopept administration has positive effects on blood glucose-insulin levels, insulin resistance and pancreas tissue in T1DM prepubertal rats. Immunohistochemical studies about pancreas and noopept usage may be useful for future studies.

Where applicable, experiments conform with Society ethical requirements