Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB237

Poster Communications

The pattern of serum microvesicles changes in preeclamptic women in comparison with controls. Analysis of their effects on renal glomerular endothelial cells and podocytes, cultured alone or in co-stimulation.

E. Grossini1, G. Raina1, S. Farruggio1, F. Pagani1, D. Surico1, M. Quaglia1, V. Cantaluppi1

1. University East Piedmont, Novara, Italy.

  • MVs size and concentration in PE and controls

  • Effects of serum (A, C) and MVs (B, D) of PE patients on renal endothelial cells and podocytes viability

In preeclampsia (PE), the release of vasoconstrictive factors and cytokines by ischemic placental tissue would be involved in endothelial dysfunction and tissue damage in many organs among which the kidney (Timofeeva et al, 2018). Also, microvesicles (MVs), that are crucial in the processes of pregnancy, could play a role in the physiopathology of PE by modulating the crosstalk between different cell types (Adam et al, 2017). About this issue, however, the exact mechanisms have not been clarified, yet. For this reason, we aimed to analyze the pattern of MVs taken from serum of PE patients and to evaluate their role as biomarkers of disease activity and biological function on renal glomerular endothelial cells (GEC) and podocytes cultured alone and in co-stimulation. MVs dimensions, concentrations and profile pattern from 20 PE patients at diagnosis, delivery and 1 month postpartum were characterized and compared with those of 20 controls. Moreover, a correlation with uricemia, proteinuria, transaminases, angiogenetic factors and arterial blood pressure was performed. The effects of serum and MVs on cell viability of GEC and podocytes cultured alone or in co-stimulation were examined, too. In vitro experiments were repeated in 5 different cell samples, at least. Statistical analysis was performed through one-way ANOVA followed by Bonferroni post hoc test or Student's t test for paired or unpaired data. For statistical significance a p<0.05 was considered. The results obtained have shown that in PE patients, MVs had higher dimensions in comparison with controls at delivery (Fig. 1). Moreover, the MVs concentration was higher in severe PE than in mild PE at delivery and at 1 month after delivery (Fig. 1). At diagnosis most MVs were PLAP +, CD24+, CD42b+ and CD105+. Both serum and MVs of PE patients caused a decrease of cell viability of GEC and podocytes, an effect which was more evident in severe PE (Fig. 2). Also podocytes treated with conditioning medium taken from GEC shown reduced cell viability, in particular when GEC were stimulated with MVs. In conclusion, in PE, serum MVs could represent a mechanism of kidney damage by modulation of the cross talk between GEC and podocytes. Our findings would be of clinical relevance and would open interesting diagnostic and therapeutic perspectives in the management of PE.

Where applicable, experiments conform with Society ethical requirements