Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB245

Poster Communications

Quercetin Prevents the Supression of Epo by TNF-alpha

M. OZKURT1, R. ÖZYURT1, A. KARADAĞ1, N. erkasap1

1. Eskisehir Osmangazi University, Eskisehir, Turkey.

  • Quercetin increased the Epo mRNA amount supressed by TNF-alpha

In-vitro studies have shown that TNF-alpha significantly lower the Epo synthesis in a dose dependent manner in HEPG2 cells (1). Quercetin is a flavanoid found in many fruits and vegetables such as oranges, grapefruits and onions which has been shown to have anti-oxidant and anti-inflammatory cell protective effect. Novel studies has shown that Quercetin increases the amount of Epo in-vitro (2). We aimed to try to remove the inhibition of TNF-alpha on Epo in with quercetin in HEPG2 cells. After detecting the non-toxic doses of TNF-alpha and Quercetin with MTT, we seeded 5*105 HEPG2 cells in 6 well plates. Each 6 well plates were a group and the groups were; no treatment group (control), 10 ng/ml TNF-alpha treated group (T), 10µM/ml Quercetin treated group (Q), TNF-alpha and Quercetin (T+Q) together treated group. After 24 hour of incubation we collected the cells and isolated total RNA, synthesied cDNA and performed Q-PCR for Epo. Data were analyzed by Kruskal-Wallis test and Dunn's test for post-hoc. TNF-alpha significantly decreased (p<0,05) the Epo mRNA amount according to control group. In Quercetin alone treated group and the combination treatment group, Epo mRNA amount were same about the control group. More detailed studies has to be done but this initial study shows that Quercetin may prevent the Epo suprression by TNF-alpha. We will try to explain the mechanism to understand the relation between Epo, TNF-alpha and Quercetin. Quercetin may be a source of treatment for anemia in chronic inflammatory diseases, as well as many other known effects.

Where applicable, experiments conform with Society ethical requirements