Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB296

Poster Communications

Effect of High Fructose Corn Syrup Consumption and Stress on TRPM2 Immunoreactivity in Rat Brain

O. Bulmus1, G. Zorlu2, Z. Ercan1, T. Kuloglu3, F. Gulcu Bulmus4, E. Kacar5, I. Serhatlioglu2

1. Physical Therapy and Rehabilitation, Firat University Faculty of Health Sciences, Elazig, Turkey. 2. Biophysics, Firat University Medical School, Elazig, Turkey. 3. Histology and Embryology, Firat University Medical School, Elazig, Turkey. 4. Vocational School of Health Services, Firat University, Elazig, Turkey. 5. Physilogy, Firat University Medical School, Elazig, Turkey.


Metastin-like Transient Receptor Potential Channel 2 (TRPM2) is a nonselective cation channel that is activated under oxidative stress conditions and leads to increased intracellular free calcium concentration and cell apoptosis. The protein channels are widely expressed in central nervous system and brain, which are quite vulnerable to oxidative stress. In this study it has been researched consumption of high fructose corn syrup (HFCS), which is quite widespread use in the food industry in recent years, and stress on the TRPM2 channels immunoreactivity in rat brain. In the study, 28 Wistar-Albino male rats were divided randomly into 4 groups and with 7 animals in each group: Group I (Control), Group II (applied a 2 weeks 20 % (v/v) HFCS solution), Group III (applied a 2 weeks 40% (v/v) HFCS solution) and Group IV (rats were exposed to a chronic restraint stress procedure: 3h/day in first week, 6h/day in second week). At the end of the study, blood samples were taken from the animals. Total oxidant (TOS) and total antioxidant levels (TAS) were measured by ELISA method (Enzyme-Linked Immunosorbent Analysis) and the oxidative stress index (OSI) was calculated. Immunohistochemistry was performed in some stress-related brain regions (prefrontal cortex, amygdala, nucleus accumbens, hippocampus). Brain samples were removed and fixed 10% formaldehyde solution. Paraffin blocks were prepared and 5 μm brain section stained with Hematoxylin-Eosin and Periodic Acid Schiff (PAS) method to analyses TRPM2 ion channel immunoreactivity. Immunohistochemical study showed that TRPM2 immunoreactivity were increased in all brain regions in HFCS (20%), HFCS (40%) and stress groups compared to control group. TOS levels and OSI values, which are reflecting oxidative stress status of body, were found to be higher in HFCS (20%, 40%) and stress groups compared to control. There was no significant difference in TAS levels, reflecting body antioxidant systems, between groups. As a result, it was seen that consumption of high fructose corn syrup may lead to oxidative stress and increased TRPM2 immunoreactivity in the brain similar to stress.

Where applicable, experiments conform with Society ethical requirements