Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB304

Poster Communications

Effect of Agomelatine on Transient Receptor Potential Vanilloid-1 Expression in Mice

M. Ozcan1, N. Ulker2, E. Etem Onalan3, A. YARDIMCI2, A. Tektemur3

1. Biophysics, Firat University Medical School, Elazig, Turkey. 2. Physiology, Firat University Medical School, Elazig, Turkey. 3. Medical Biology, Firat University Medical School, Elazig, Turkey.

The transient receptor potential vanilloid type-1 (TRPV1) is a nonselective cation channel that is considered to be an important activator of various pain stimuli such as exogenous and endogenous physical and chemical stimuli. TRPV1 is high levels expressed in the central nervous system and play an important role in pain mediated by central sensitization. This work presents new data concerning the effect of agomelatine, a new antidepressant, on expression of TRPV1 in periaqueductal gray (PAG), raphe nucleus, amygdala and nucleus accumbens (NAc) of healthy and diabetic mice. Agomelatine was administered for acute and chronic (15 consecutive days (twice per day)) in healthy and diabetic mice (n=7 for each group). Diabetes was induced in mice by intraperitoneal (i.p.) injection of streptozotocin (STZ). Agomelatine (10mg/kg) treatment was performed six weeks after STZ injection in diabetic groups. Expression of TRPV1 in PAG, raphe nucleus, amygdala and NAc were detected by real time-PCR. Agomelatine administration caused significant changes in TRPV1 mRNA levels in amygdala, PAG and raphe nucleus in healthy and diabetic mice (p<0.05, p<0.01). The findings suggest that agomelatine change TRPV1 expression in diabetic mice. Agomelatine may have exerts its beneficial effects on pain transmission and affective disorders via TRPV1. Key words: Agomelatine;

Where applicable, experiments conform with Society ethical requirements