Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB328

Poster Communications

Investigation Effects of Metformin in rats ileum contractility

S. Sahinturk1, A. YARDIMCI2, N. Ulker2, I. Serhatlioglu3, E. Kacar2

1. Department of Physiology, Uludag University, Bursa, Turkey. 2. Department of Physiology, Firat University, Elazig, Turkey. 3. Department of Biophysics, Firat University, ELAZIG, Turkey.

S. Sahinturk1, A. Yardimci2, N. Ulker2, I. Serhatlioglu3, E. Kacar2 1. Department of Physiology, Faculty of Medicine, Uludag University, Bursa, Turkey 2. Department of Physiology, Faculty of Medicine, Firat University, Elazig, Turkey 3. Department of Biophysics, Faculty of Medicine, Firat University, Elazig, Turkey Type 2 diabetes mellitus (T2DM) is among the most common disorders at world and affects peoples daily-life adversely. Metformin is one of the most effective agent for treatment of T2DM around worldwide. It is an antihyperglycemic medication, which improves glucose tolerance in patients with T2DM, decreasing both basal and postprandial plasma glucose levels. Metformin has some adverse effects, most common is gastrointestinal symptoms, including vomiting, nausea, diarrhea, stomachache, loss of appetite and indigestion. One of the most important these effects is diarrhea, because it can lead to disruption of patient adaptation to the treatment. Because of metformin cause to decrease intestinal glucose absorbtion, so it may result in diarrhea. However, effects of metformin on rat intestinal contractility is almost unknown and these effects may contribute to the diarrhea. In this study, we investigated metformin effects on rat ileum contractility in isolated tissue bath. Adult female Sprague-Dawley rats (n=8) were used in this study. In order to investigate of metformin effects on ileum conractility, ileum (nearly 10 mm in length) was quickly removed from 5 cm proximal to the ileocecal valve, placed in Krebs solution and then mounted in (tension 1 g) isolated tissue baths (5, 10 and 20 mL) bubbled with 95 % O2 and 5 % CO2 at 37°C. The tissues were equilibrated for one hour with rinsing every 15 min under a resting tension of 1 g before starting experiment. The tension formed by the tissues was measured using transducers MP150 instrument (Biopac Systems, Inc., U.S.A.). After equilibration, acetylcholine (10-4 M) was given to the all baths. After one hour rinsing, metformin (2 mM) was given and incubated 15 min. After 15 min, again acetylcholine was given same dose without rinsing and then these part was repeated with metformin 20 mM dose. Control group values were accepted as 100 %, and change calculated as percent (%) for all treatment groups for peak to peak amplitude and area values. Data were given as means ± SE and statistical analysis of the results was implemented by one way ANOVA. In all conditions, differences were considered significant when p<0.05. Area values compared to control group, there was no significant changes at any doses. However, peak to peak amplitude values compared to control group, there was significant decrease in 2 mM (73.68±10.21, change %, p<0.01) and 20 mM (76.83±5.48, change %, p<0.05). As a result, it was concluded that metformin may reduce acetylcholine-induced contractile activity in rat ileum.

Where applicable, experiments conform with Society ethical requirements