Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB340

Poster Communications

Extra virgin olive oil phenol, hydroxytyrosol, vasodilates uterine arteries in an estrogen receptor- and nitric oxide-dependent manner

L. Barberio1, R. D' Agostino1, M. Mandala1

1. Biology, Ecology & Earth Sciences, University of Calabria, Rende (CS), Italy.

Extra Virgin Olive Oil (EVOO), a major ingredient of the Mediterranean diet, has been shown to confer benefits to human health by protecting against cardiovascular disease. Special attention has been on phenols (a component of EVOO) which have been shown to produce vasodilation in some type of vessels, although the underlying mechanisms remain unknown. Phenols have not been tested on uterine arteries, which must vasodilate and grow during pregnancy to increase uteroplacental blood flow to meet fetal demands. These processes are compromised in some gestational diseases such as preeclampsia and fetal growth restriction. In this study, we tested the effects of EVOO phenols on isolated, pressurized rat uterine (UA) and mesenteric (MA) resistance arteries. The contribution of the two major EVOO phenols - Hydroxytyrosol (HT) and Oleuropein (Ole) was examined, and the underlying mechanisms probed by using L-NAME and ICI182,780 to inhibit nitric oxide synthase (NOS) and estrogen receptors (ERs) respectively. Both HT and Ole induced vasodilation of UA in a concentration-dependent manner. At the highest concentration (3x10-5 M), the vasodilation was 85,7 ± 8% by HT and of 26,3 ± 6% by Ole. Neither compound had any effect on MAs. UA vasodilation to HT was both ER- and NO-dependent; conversely, inhibition of ERs or NOS did not affect UA vasodilation to Ole, indicating that other, as yet unrecognized mechanisms are responsible.

Where applicable, experiments conform with Society ethical requirements