Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB360

Poster Communications

In vitro effects of alarin on human saphenous vein contraction

R. Ozen Koca1, A. Koc3, H. Solak1, Z. Solak Gormus1, F. Taki1, N. Gormus2, S. Kutlu1

1. Department of Physiology, Necmettin Erbakan University Meram Faculty of Medicine, Konya, Turkey. 2. Department of Cardiovascular Surgery, Necmettin Erbakan University, Meram Faculty of Medicine, Konya, Turkey. 3. Physiology, Hitit university Medicine Faculty, Corum, Turkey.

AIM: Alarin is a novel endogenous vasoactive neuropeptide. Oriented information of vascular effects of alarin has been obtained from micro vascular dermis vessels. In this study we aimed to investigate the effects of alarin on human saphenous vein muscle contractility. MATERIAL AND METHODS: Human saphenous vein (n=28) were taken taken from patients who underwent coronary bypass surgery. The patients' ages were between 47 to 72. All samples were cleaned and sectioned into 3-4 mm long rings. Rings were placed in organ baths containing Krebs solution, thermoregulated at 37oC and aerated (95 % O2 and 5% CO2). Changes in isometric tension of rings were recorded by using a four channel force displacement transducer. All tissues washed for 3 hours in order to diminish the effects of anaesthetic agents. Phenylephrine (PE 10-6 M) was administered in tissue chamber for producing isometric contractions. Amplitudes of contractions were determined and used for statistical analyses. Cumulative alarin (10-8 to 10-5) doses were added in organ baths. The contractions were recorded accordingly. Friedman and Kruskal Wallis tests were used for statistical evaluation. RESULTS AND CONCLUSIONS: Alarin treatments at 10-8, 10-7, 10-6 doses had no significant changes on muscle contractility. But alarin inhibited the contraction at 10-5 M concentration dose following PE administration (p<0.05). This effect of alarin occured in a dose dependent manner. These findings have a potential to contribute to studies owing to effect of alarin on cardiovascular system. Further studies are needed to clarify the mechanism(s) of alarin.

Where applicable, experiments conform with Society ethical requirements