Proceedings of The Physiological Society

Europhysiology 2018 (London, UK) (2018) Proc Physiol Soc 41, PCB362

Poster Communications

Effects Of Alarin In Healty And Damaged Rat Aorta

H. Solak1, Z. Solak Gormus1, R. Ozen Koca1, A. Koc3, F. Taki1, N. Gormus2, S. Kutlu1

1. Meram faculty of medicine department of physiology, Necmettin erbakan University, Konya, Konya, Turkey. 2. Meram faculty of medicine department of cardiovascular surgery, Necmettin erbakan University, KONYA, Turkey. 3. physiology, Hitit University Medicine Faculty, Çorum, Turkey.

AIM: The new peptide alarin, a member of galanin neuropeptide family as well, probably has effects on different receptors except galanin receptors. So it can possibly reveal both similar effects like galanin neuropeptide family and exactly converse effects. Therefore determination of effects of endogenous peptide alarin on vessels is important for contributing physiology and also pathophysiology of smooth muscle related cases like ever increasing hypertension, at the present time enables scientists to obtain new symptoms. Their effects on vessels are not investigated experimentally. We aimed to investigate the effects both on healthy and damaged rat aorta. MATERIAL AND METHODS: Wistar albino rats divided into 2 groups (n=24). Group 1 aorta-intact endothelium (n=12), group 2 aorta-damaged endothelium (n=12). Descending thoracic aorta was isolated after cervical dislocation. Aorta tissues were cleaned and sectioned into 3-4 mm long rings. Rings were placed in organ baths containing Krebs solution, thermoregulated at 37oC and aerated (95 % O2 and 5% CO2). Changes in isometric tension of aorta rings were recorded using a four channel force displacement transducer. Phenylephrine (PE 10-6M) was applied and contractions were recorded in both groups. Then alarin was given cumulatively (10-8-10-5M) to group 1. In group 2 aorta damage was achieved by tearing endothelium with needle. After controlling endothelial damage by applying acetylcholine (Ach 10-6M), damaged strips were washed for one hour and second dose of PE was administered and then alarin was given cumulatively to group 2, contractions were recorded. RESULTS AND CONCLUSION: After alarin was given cumulatively to group 1, significant inhibition of spontaneous contractions was noticed in alarin doses of 10-8-10-5 (p<0.05). Alarin 10-8-10-5 also made significant inhibition in contractions when compared to 10-7-10 -6. Group 2 inhibition of second PE contractions continued after alarin doses, but it was less significant when compared with group 1 (p<0.05). These results suggest that alarin may have beneficial effects on treatment of hypertensive disorders. Further studies were needed to clarify the mechanism of alarin.

Where applicable, experiments conform with Society ethical requirements