An Introduction to Molecular Biology

23 April 2019 - 26 April 2019

Cruciform Building, University College London,
Gower Street,
London WC1E 6BT, United Kingdom

Organised by Caroline Pellet-Many, Royal Veterinary College, and Laura Wisniewski, University College London, UK

This workshop is intended for physiologists who want to learn the basic yet essential techniques used in molecular biology. It will also illustrate the principles and methodologies behind the manipulation of protein expression using a variety of transfection techniques. 26 places are available on this course. 

Please note that the course is now full. For more information, please contact Laura Wisniewski.

Techniques covered will include:

  • Gel electrophoresis and visualisation of DNA
  • Restriction enzyme digest, DNA fragment size estimation and mapping
  • Bacterial transformation
  • Mini‐prep (DNA extraction)
  • RNA isolation and cDNA reverse transcription
  • PCR (polymerase chain reaction)
  • Transfection
  • siRNA knock‐down
  • EGFP analysis
  • Western blot
  • Protein quantification

There is no charge for this workshop, although delegates are expected to organise and fund their own accommodation. 

Members of The Physiological Society are encouraged to apply for travel grant funding to support their attendance on this course.