Salmonella enterica sv. Typhimurium has developed a sophisticated way of communicating with its mammalian host to ensure its survival and multiplication throughout the process of infection. Although many Salmonella genes have been shown to be required to cause disease, little is known about host cell type specificity of Salmonella gene expression. In order to address this question we have used an integrative approach that combines DNA microarrays and a reporter system based on single copy gfp+ transcriptional fusions to determine the transcriptional profile of intracellular S. Typhimurium following infection of epithelial HeLa cells. Comparison of the Salmonella response to macrophage intracellular environment [Eriksson et al., 2003, Mol. Microbiol. 47: 103-118] showed that this pathogen has a distinct expression profile during infection of human epithelial cells. We have new evidence that the SPI1 and SPI2 pathogenicity islands are differentially expressed in macrophages and epithelial cells. Furthermore, we observed that Salmonella can escape the vacuolar compartment and multiply in the cytosol. This multiplication is accompanied by production of flagella within the epithelial cells. We will describe the major differences observed between the Salmonella response to macrophages and epithelial cells.
University of Newcastle (2004) J Physiol 559P, C4
Communications: The extravacuolar life of an intravacuolar pathogen: salmonella can escape the vacuolar compartment of human epithelial cells
Hautefort, Isabelle; Thompson, Arthur ; Eriksson, Sofia ; Parker, Mary; Proenca, Maria Jose; Rhen, Mikael ; Hinton, Jay C.D.;
1. Molecular Microbiology Group, Institute of Food Research, Norwich, United Kingdom. 2. Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden. 3. Faculdade de Medicina de Lisboa, Lisbon, Portugal.
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