Modulation of blood components, both red blood cells and plasma, particularly affects on circulatory system. This study investigated the load-dependent cardiac function and systemic changes after normovolemic hemodilution with four different transfusion fluids in anesthetized Golden Syrian hamsters. The investigation was performed in anesthetized 60-70 g male Golden Syrian hamsters. Animal handling and care followed the US National Institute of Health Guide for Care and Use of Laboratory Animals. Twelve hamsters were randomly divided into 4 groups (n=3 for each group). Anesthesia was induced by intraperitoneal injection of sodium pentobarbital (50 mg/kg) and core body temperature was maintained using a heating pad. Animal surgical preparation included: (i) left jugular vein for transfusion fluid injection, (ii) right common carotid artery for a pressure-volume conductance catheterization, (iii) left femoral artery for blood withdrawal, and (iv) tracheotomy with polyethylene-90 tube to facilitate spontaneous breathing. Normovolemic hemodilution was performed by the exchange of 40% of the animal’s blood volume with transfusion fluid. Four different transfusion fluids included 3-week stored blood, dextran 70 kDa (viscosity 3.0 cP), low viscosity plasma volume expander (LVPE; 2% dextran 2000 kDa, viscosity 2.2 cP), and high viscosity plasma volume expander (HVPE; 6% dextran 2000 kDa, viscosity 6.4 cP). Left ventricular cardiac systolic function was quantitatively assessed with a pressure-volume conductance catheter. Values are means ± S.D., compared by ANOVA. Plasma viscosity after hemodiluted with HVPE was 2.12±0.10 CP after hemodilution while other groups were 1.01±0.10 (stored blood), 1.27±0.03 (dextran 70 kDa) and 1.21±0.05 cP (LVPE). Cardiac output at the end of experiment increased from baseline in the group hemodiluted with HVPE (10.35±2.38 ml/min at baseline vs 13.10±4.02 ml/min at the end of experiment) and dextran 70 kDa (10.685±2.62 ml/min at baseline vs 12.49±1.64 ml/min at the end of experiment). On the other hand, cardiac output in the group hemodiluted with LVPE was less than baseline (13.11±0.49 ml/min at baseline vs 10.96±0.91 ml/min at the end of experiment) that was similar to the group exchanged with stored (8.40±3.49 ml/min at baseline vs 6.76±2.85 ml/min at the end of experiment). Stroke work relative to baseline in the group hemodiluted with HVPE significantly increased compared to those in the group hemodiluted with LVPE (HVPE: 1.23±0.14 vs. LVPE: 0.81±0.15, p<0.05). These data suggest that modulation of plasma expander viscosity affects load dependent cardiac function. Stored blood reduces load dependent cardiac function when compared to baseline condition.