Pyrethroids are important insecticides used largely because of their high activity as an insecticide and their low mammalian toxicity. The present study was designed to investigate the propensity of permethrin to induce oxidative stress and biochemical perturbations in rat liver and the role of vitamin C in alleviating its toxic effects. Male Wister Albino rats were randomly divided into four groups of 7 each, group I served as control; group II treated with vitamin C (200 mg/kg BW), group III received permethrin (150 mg/kg BW) and group IV treated with permethrin plus vitamin C. Rats were orally administered their respective doses daily for 28 days. The local committee approved the design of the experiments, and the protocol conforms to the guidelines of the National Institutes of Health (NIH). The administration of permthrin significantly caused elevation in lipid peroxidation (LPO) and reduction in the activities of antioxidant enzymes including catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione peroxidase (GSH-Px) and glutathione reductase (GSH-Rx). A significant decrease in glutathione (GSH) content was also observed. Liver aminotransferases (AST and ALT) and alkaline phosphatase (AlP) were significantly decreased while lactate dehydrogenase (LDH) was increased. Vitamin C treatment to permethrin intoxicated rat decreased LPO level and normalized CAT, SOD, GST, GPx and GR activities, while GSH content was increased. Also, liver AST, ALT, AlP and LDH were maintained near normal level due to vitamin C treatment. In conclusion, vitamin C has beneficial effects and could be able to antagonize permethrin toxicity.