The present study was designed to investigate the impact of abamectin to induce oxidative stress and biochemical perturbations in male albino rat (Rattus norvegicus) serum and the role of vitamin C and vitamin E in ameliorating its toxic effects. Rats were randomly divided into 6 groups of 10 each, group I served as control; group II treated with vitamin C (VC; 20 mg/kg BW), group III treated with vitamin E (VE; 200 mg/kg BW/day), group IV received single dose of abamectin (2.175 mg/kg BW/day, 1/4 LD50), group V treated with abamectin plus vitamin C and group VI treated with abamectin plus vit E. Rats were orally administered their respective doses of VC and VE daily for 10 days. The local committee approved the design of the experiments, and the protocol conforms to the guidelines of the National Institutes of Health (NIH). After 10 days of treatment, serum aspartate amino transferase (AST), alanine amino transferase (ALT), gamma-glutamyl transferase (GGT) alkaline (AlP) and acid phosphatase (AcP) as well as malondiadehyde (MDA), glutathione (GSH), glutathione-S-transferease (GST), catalase (CAT) and superoxide dismutase (SOD) were measured. The results revealed significant increase in serum AST, ALT, AlP and AcP activities in abamectin group as compared to the control. Also, the level of MDA was significantly increased in abamectin treated rats. On the other hand, abamectin induced a significant inhibition of GST, CAT SOD and GSH contents as compared with the control group. Supplementation of vitamin C and vitamin E to abamectin – treated rats reduced the toxic effect of abamectin on the above measured parameters and lipid peroxidation in serum. In Conclusion, the findings data suggested that administration of vitamins C and E ameliorated the abamectin -induced oxidative stress in albino rats and the effect of vitamin E in preventing abamectin -induced toxicity was superior to that of vitamin C.