The role of adrenergic receptors in nicotine-induced hyperglycaemia has not been well studied in amphibians. Thus, this study investigated the effects of alpha and beta adrenergic receptor blockers in nicotine-induced hyperglycaemia in the common African toad Bufo regularis. Each toad was fasted for 24hr and anaesthetized with sodium pentobarbitone (3mg/100g i.p). The animals received intravenous (i.v) injection (abdominal vein cannula) of 0.7% amphibian saline, or nicotine (50μg/kg) and thereafter, blood samples were collected for estimation of blood glucose level. In the treated groups, the toads were pretreated with prazosin (0.2mg/kg i.v), propranolol (0.5mg/kg i.v) or prazosin (0.2mg/kg i.v) combined with propranolol (0.5mg/kg, i.v) 30 minutes before i.v. injection of nicotine (50μg/kg i.v). Thereafter, blood samples were also collected from truncus arteriosus for estimation of blood glucose level using the modified glucose oxidase method. Nicotine caused significant increase (P<0.01) in the levels of blood glucose in the common African toad. Pre-treatment of the toads with prazosin (0.2mg/kg i.v) or propranolol (0.5mg/kg, i.v) significantly (p < 0.01) reduced the hyperglycaemia induced by nicotine (50μg/kg i.v). However, the combination of prazosin (0.2mg/kg i.v) and propranolol (0.5mg/kg, i.v) abolished the hyperglycaemic effect of nicotine (50μg/kg i.v). The results of this study suggest that both alpha and beta adrenoceptors are involved in nicotine-induced hyperglycaemia in common African toad Bufo regularis.