Inflammation is a complex biological response involved in pathogenesis of a variety of diseases. Although steroids and NSAIDs are the main therapeutic agents in inflammation they cause serious side effects. Therefore, the development of new materials with comparable results and no side effects is needed. The purpose of our study is to investigate the biological activity of materials based on gold (Au-VO) and silver (Ag-VO) nanoparticles and natural compounds extracted from European cranberry bush – Viburnum opulus L. (VO) on acute inflammation model in Wistar rats. The inflammation was induced by intraplantar injection of 100 μl 1% carrageenan and the response was compared to indomethacin (5 mg/kgc) as positive control and saline solution as negative control. The VO extracts (15 and 30 mg/kg b.w.) and the materials based on gold and silver nanoparticles functionalized with VO extract (0.3 mg/kg b.w.) were administrated orally during 4 days before injection of carageenan. At 2h, 24h and 48h after carageenan injection the paw edema were measured and plantar tissue were taken for histopathological and immunohistochemical investigations (iNOS and COX-2) and to evaluate malondialdehyde (MDA), glutathione reduced/glutathione oxidized (GSH/GSSG), IL-1 and IL-6 levels. In addition, catalase and glutathione peroxidase activities were assessed from plantar tissue and erythrocyte lysates. Values are means ± S.E.M., compared by ANOVA and Tukey posttest. Low dose of VO significantly inhibited edema formation (44.54% vs. control group treated with vehicle; p<0.05), particularly at 2h, and increased GSH levels, both at 2h (2.98±1.63 nmoles/mg protein; p<0.01) as well as at 48h (21.66±14.36 vs. 7.01±1.34 nmoles/mg protein in control group; p<0.0001). The inhibition was higher to those produced by indomethacin (22.52%) and Ag-VO (22,52%) and was maintained at 24 h and 48 h, but at lower levels. In parallel, IL-1 and IL-6 levels and iNOS expression decreased (p<0.01). Ag-VO and Au-VO remarkably inhibited generation of MDA (0.13±0.09 respectively 0.11±0.04 vs. 0.29±0.12 nmoles/mg protein; p<0.05) and GSSG (1.79±0.75 respectively 1.85±0.46 vs 3.52±2.09 nmoles/mg protein in control group; p<0.001) in the plantar tissue, decreased IL-1 and IL-6 levels and increased GPx activity in paw tissue (89.25±10.90 respectively 86.25±17.06 vs 38.00±12.11 U/g protein in control group). This findings suggested that the nanoparticles functionalised with natural compounds may be useful in the treatement of inflammatory disease.