The study of the relationships between transcutaneous variables, especially microcirculatory flow by Laser Doppler Flowmetry (LDF), transcutaneous gas pressures (tcpO2 and tcpCO2) and transepidermal water loss (TEWL) has provided new directions to better understand local circulatory physiology. The objective is to explore how changes in local perfusion in the lower limb affect the epidermal “barrier” function. A group of fourteen young adult males (24.9 ± 0.6 years old), was selected after informed consent. All procedures complied with the ethical standards for human research by the Declaration of Helsinki and subsequent amendments. Two protocols were designed involving postural change maneuvers (protocol I – elevation of the leg 90° relative to the body axis while sitting; protocol II – supine with elevation of the leg 30°) and one protocol consisting of an occlusion test with application of suprasystolic pressure through a tourniquet-cuff (protocol III). Studied variables in distal locations of the lower limb involved microcirculatory blood flow by LDF (PeriFlux PF5000, PF5010 System, Perimed, Sweden), tcpO2 by transcutaneous gasimetry (Periflux PF5000, PF5040 System, Perimed, Sweden) and TEWL (Tewameter TM300, CK electronics, Germany). All variables were assessed before, during and after these maneuvers. Descriptive and nonparametric statistics were applied and a 95% confidence level was adopted.In Protocol I there was a statistically significant increase in the values of LDF and TEWL and an equally significant decrease in tcpO2 during the elevation of the lower limb. When the lower limb returned to the starting position values returned to baseline. In Protocol II there was a statistically significant decrease of LDF values, TEWL and tcpO2 during the elevation of the lower limb. The values returned to baseline when the lower limb returned to the starting position. In Protocol III there was a statistically significant decrease in the values of LDF and tcpO2 during the occlusion, accompanied by a significant increase of TEWL values, both of which returned to baseline when the tourniquet was removed. The present data confirms previously published results, suggesting that by modifying local perfusion conditions one may influence the epidermal skin “barrier”.