Mechanisms underlying generation of spontaneous myometrial contractions are poorly understood. Although morphological evidence indicates existence of interstitial cell of Cajal-like cells in myometrium it remains unclear whether these cells are involved in uterine pacemaking. The “funny” (If) current (or funny channel or pacemaker current), originally described in sinoatrial node and Purkinje fibers, are currently investigated for their potential role(s) in smooth muscles with generation of spontaneous phasic activity. The aim of this study was to obtain pharmacological evidence for the possible role of funny ion channels in spontaneous and agonist – stimulated contractions in isolated rat myometrium using the pharmacological blocker ivabradine. Myometrial strips were removed from nonpregnant rats and placed in an organ bath containing physiological salt solution at 37°C and pH 7.4, constantly bubbled with 95% oxygen-5% carbon dioxide and isometric contractions were recorded. Effects of cumulative concentrations of ivabradine (1, 3 and 10 uM) on amplitude, area-under contractile curve and frequency of spontaneous and oxytocin-and prostaglandin F2 alpha-induced contractions were studied by 10-minutes intervals. Statistical analysis was performed using one -way ANOVA and post-hoc tests were performed with Tukey HSD. Ivabradine (at any cumulative concentration) did not have any significant effect on either contractile parameter (amplitude, AUC or frequency) of spontaneous (n=6) and agonist induced contractions stimulated by oxytocin (n=7) or prostaglandin F2alpha (n=7). Results from this in vitro study involving myometrial strips from nonpregnant rat indicates that, funny currents does not have any significant role(s) in spontaneous contractions and nor they involve in agonist-induced phasic contractions.