Purpose: The purpose of this study was to examine the correlation between functional modulation of the autonomic nervous system and the menstrual cycle.Methods: Subjects were comprised of 11 healthy female college students (19.5±0.5 years old) with regular menstrual cycles. The changes in emotional sweating induced by hand work (beans work and puzzle) and the modulation in autonomic nervous system(electrocardiograph and heartbeat) with active orthostatic load were compared among the follicular, luteal and menstrual phases. The Japanese version of the Profile of Mood State (POMS), healthy life habit (DIHAL.,2,junior high school student~adult) were also examined. Analysis: Effect size of the difference between group or correlation were evaluated. Results:Emotionalsweating( task is beans work) during the menstrual phase was significantly higher than those during follicular and luteal phases and it associated with the high score of “depression dejection” of POMS factors. Group with the highest T score (standardized score) of “depression dejection” emotional sweating was significantly higher during luteal phase. In addition, the group which complained subjective low healthy state, showed higher emotional sweating (beans work) during luteal phase.Fluctuations of heart rate at rest(the size of the activity) ,during menstrual phase was significantly higher than those during follicular phase, although it was a normal range.The balance of the autonomic nervous activity at rest (balance) was significantly higher during luteal phase compared to menstrual phase, indicating predominance of sympathetic nervous system. Active orthostatic load-induced fluctuations of heart beat(powers of the response)was significantly higher during follicular phase compared to those during menstrual and luteal phase, although it was a normal range. Fluctuations of balance of the active orthostatic load (powers of the change) was significantly higher during menstrual phase than that during follicular phase; the range which need special medical attention. In addition, there found an association between balance and “confusion”. Group with the highest T score of “confusion” factors, showed predominant sympathetic nervous system during menstrual phase.Conclusion:These findings suggest that Stresses of beans work may influence the function of the autonomic nervous system to during menstrual phase . In addition, these findings suggest that powers of the change was higher during menstrual phase, the range which need special medical attention. Students with the high T score of “confusion” factors, showed the balance is sympathetic nerve predominance during menstrual phase.