A critical feature of brain plasticity is the capacity to dynamically adapt in response to the environment by remodeling connections between neurons. Inhibitory neurons are known to play a vital role in defining the window for critical period plasticity during development, and it is increasingly apparent that they continue to exert powerful control over experience-dependent cortical plasticity in adulthood. Recent in vivo imaging studies demonstrate that long-term plasticity of inhibitory circuits is manifested through structural rearrangements. Changes in sensory experience drive this structural remodeling of inhibitory interneurons in a cell type and circuit specific manner. We recently found that inhibitory synapse formation and elimination occurs with a great deal of spatial and temporal precision, and is often locally coordinated with excitatory synaptic changes on the same dendrite, largely clustered within a 10 um distance. Yet, the nature of the clustered inhibitory and excitatory synaptic dynamics remains temporally unresolved in terms of whether the two events occur simultaneously or if one of the two events drives the change, while the other adjusts to it. Does the presence of a dynamic inhibitory synapse destabilize neighboring spines, or do excitatory synaptic changes drive inhibitory synapse dynamics? Are the valence changes of coordinated events weighed in the same direction, or are they compensatory? I.e. does excitatory synapse loss drive formation or loss of an inhibitory synapse? To further probe the nature of ‘coordination’ between inhibitory and excitatory synaptic dynamics we triple-labeled L2/3 pyramidal neurons in the mouse visual cortex via in utero electroporation using YFP as a cell fill, PSD95-mcherry to label excitatory synapses, and Teal-Gephyrin to label inhibitory synapses. Upon reaching adulthood, these mice were implanted with cranial windows and labeled cells in binocular visual cortex were imaged in vivo at short intervals using spectrally resolved two-photon microscopy.