The rodent hippocampus can be divided into dorsal and ventral domains based on distinct anatomical, biochemical, and behavioral phenotypes associated with each region. Recently, we explored the cellular properties of CA1 pyramidal neurons in these two regions, and found that CA1 neurons from the ventral region are intrinsically more excitable than their dorsal counterparts. Among the many possible differences in ion channel expression and function that could account for these different cellular properties, we have initially focused on the hyperpolarization-activated cation-nonselective current (Ih). Measurement of Ih using cell-attached patches along the somatodendritic axis revealed a significant distant-dependent depolarizing shift in the V1/2 of activation for h-channels in ventral, but not dorsal neurons. Ultrastructural immuno-localization of HCN1 and HCN2 h-channel subunits revealed a similar distant-dependent shift in the HCN1/HCN2 ratio for ventral, but not dorsal neurons. These observations suggest that a shift in the expression of HCN1 and HCN2 drives functional changes in Ih for ventral neurons, and could thereby significantly alter dendritic integration in neurons from these two regions.