Voltage gated sodium channels (NaV channels), of which there are multiple members (NaV1.1-1.9), play an essential role in the initiation and propagation of action potentials in neurons and other electrically excitable cells. There is considerable evidence that drugs able to specifically act on these channels could have numerous clinical uses, such as in the development of pain relieving drugs and the treatment of epilepsy. Screening for inhibitors and modulators is typically achieved using mammalian cells stably expressing specific NaV channels. We have developed a range of cell lines expressing NaV channels that overcome common limitations experienced when using stable mammalian cell lines, such as slow growth rates and low levels of expression. We show high through-put fluorescent assay data and confirmatory electrophysiological data to validate our alternative approaches.
Life Sciences 2007 (2007) Proc Life Sciences, PC200
Poster Communications: Development of tools for high-throughput screening of voltage gated sodium channels
L. C. Gibson1, I. McPhee1, J. Kewney1, P. Quinn1, S. Joyce1, A. Nunn1, C. Darroch1, C. Dick1, S. J. MacKenzie1
1. Scottish Biomedical, Glasgow, United Kingdom.
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