SRC splice variants are multi-domain nonreceptor tyrosine kinases. C-src – which is found almost in all types of tissue – has been a subject of investigation for years. Alternatively, the neuronal splice variants N1 SRC and N2 SRC, also called neuronal SRCs, are not as well characterized in terms of their neuronal function. N1 src has six extra amino acids in its SH3 domain, while N2 SRC has 18 extra amino acids. We have embarked on a project to identify the interaction partners of the neuronal splice variants of SRC, N1 and N2. This has involved generating both SH3 domain and the full length SRC splice variants and determining their binding partners in lysates from presynaptic nerve terminals. We have identified a number of selective interactions of N1 and N2 by MALDI-tof mass spectroscopy. A number of these proteins have essential roles in synaptic vesicle recycling. The next step will be to determine whether their interactions occur in vivo, and which of the binding proteins are also SRC substrates. The results suggest that the SRC neuronal splice variants might have specific roles in the synaptic terminal and implicates them in a putative role in the synaptic vesicle life cycle.