Somatotype is one of the basic classifications of physical characteristic and body type. Three components were identified in the classical anthropometric somatotype Metod of Heat and Carter: relative fatness (endomorphy), musculoskeletal component (mesomorphy), and linearity (ectomorphy). As Grasgruber states (2008), the average somatotype of male European population is largely based on study of University students and is around 3.5- 4.5-3.0. Pavlik’s measurements (2003) are not different; his average somatotype of students is 4-4-3. The aim of this study was to achieve a somatotypological comparison of male and female Physical Education students. Physical parameters of students were measured using standardized anthropometrical methods and somatotypes were counted in line with the Heath-Carter Somatotype Method. A total 246 students (153 males and 93 females) participated in this study. Participants were students of the Faculty of Sport in the Czech Republic and Portugal. 63 Czech men (20-22 years old, 181.4±6.8cm, 77.3±9.4kg, BMI 23.9±2.7). 90 Portuguese men (18-21 years old, 176.1±6.2cm, 74.7±9.6kg, BMI 24.3±2.8). 58 Czech Women (20-22 years old, 169.0±6.7cm, 63.0±7.2kg, BMI 22.0±1.9). 35 Portuguese women (18-21 years old, 163.2±6.8cm, 55.3±8.5kg, BMI 20.6±2.2). To determine the differences in somatotype between the Czech and Portugal male and female students, Shapiro-Wilk W test was used. The level of significance was set to 0.05. Mean female Czech students somatotype was classified as Central type (Balanced somatotype) – (3.2±0.8-3.6±1.2-3.0±1.0), while a Balanced endomorph was registered for Portuguese female students (4.4±1.1-3.0±0.9-3.3±1.1). Mesomorphy component was higher in Czech female students than in Portuguese females students (p=0.037), endomorphy component was higher in Portuguese female than Czech female students (p=0.000) and ectomorphy component was statically non-significant between our two groups (p=0.162). Mean male Czech students somatotype was classified as ectomorphic mesomorph (2.2±0.8-4.7±1.2-3.1±0.9), while a mesomorph-endomorph was registered for Portuguese male students (3.9±1.4-4.2±1.1-2.6±1.1). Mesmorphy component was higher in Czech male students than in Portuguese males students (p<0.05), an endomorphy component was higher in Portuguese male than Czech male Students (p<0.05) and an ectomorphy component was higher in Czech male students than Portuguese students (p<0.05). Significant somatotypic differences between body structure of Czech and Portuguese male and female students of Faculties of Sport Studies. Whether these differences are caused by more theoretical bachelor programs, by differences in attitude towards sport in central and south-west Europe or by other influences.