Introduction High-intensity interval training (HIT) has been shown to increase insulin sensitivity over two weeks and six sessions of training (1-2). Adaptations in skeletal muscle have been reported previously (3-5), but it is not known whether HIT affects glucose metabolism in abdominal tissues. Methods We measured glucose uptake in skeletal muscle, liver, pancreas and visceral fat tissue using FDG PET -method during euglycaemic hyperinsulinaemic clamp in eight healthy, sedentary, middle aged men (mean ± SD, age: 47 ± 5 years, BMI: 26 ± 2.9 kg×m-2, VO2max: 34 ± 4 ml×kg-1×min-1) pre and post two weeks and six sessions of HIT (Gibala method: 4-6 × 30 s repeated Wingate tests with 4 minutes of recovery). In addition, oral glucose tolerance test was performed. Results Two weeks of HIT increased VO2max by 4.7 % (from 34 ± 4 to 35.5 ± 4 ml×kg-1×min-1, student paired t-test, p=0.019). Training had no effect on fasting plasma glucose and insulin levels, but there was a tendency for reduced serum free fatty acid concentration (pre: 0.46 ± 0.14 vs. post: 0.33 ± 0.09 mmol×l-1, p=0.054). HIT training increased insulin stimulated glucose uptake in m. quadriceps femoris by 38 % (from 44 ± 11 to 60 ± 18 µmol×kg-1×min-1, n=5, p=0.004), but had no significant effect on whole body insulin sensitivity (pre: 39.1 ± 11.4 vs. post: 43.4 ± 16.3 µmol×kg-1×min-1, p=NS) and no influence on glucose uptake in liver (24.7 ± 11.5 vs. 25 ± 11.3 µmol×kg-1×min-1, n=6, p=NS), pancreas (26.6 ± 5.1 vs. 26.1 ± 6.7 µmol×kg-1×min-1, n=6, p=NS) and visceral fat tissue (9.9 ± 5.2 vs. 8.7 ± 5, µmol×kg-1×min-1, n=6, p=NS) in healthy men. Conclusion We conclude that although HIT is a promising exercise method for health, our results suggest that further investigation is needed to see if low-volume intermitted training improves insulin activity and metabolic control on whole body level, or exclusively in skeletal muscle.