Basolateral amygdala (BLA) has been implicated in the regulation of food intake apart from hypothalamic centres(1). In the present study, we hypothesized that the Orexin B, a polypeptide discovered in the lateral hypothalamic region, may be involved in modification of role of amygdaloid centres(2). Therefore we studied the effect of bilateral infusion of Orexin B and its antagonist (TCS-OX2-29) into BLA to study the food and water consumption. Adult male Wistar albino rats (n=54) were selected and grouped into (i) Orexin infusion – Low dose; (ii). High dose (3 ng/kg, 30 ng/kg, on the basis of previous studies(3,4) (iii). TCS-OX2-29 infused group. Each group was subdivided into control, sham operated control and experimental subgroups (n=6 each). Fasted animals were infused with Saline Or Orexin B OR TCS-OX2-29 (as the case may be) into BLA bilaterally by implanting a cannula using stereotaxic method. Harvard Pico Plus infusion pump was used for microinfusion of these chemical agents. Following infusion, sequential Food intake and water intake were measured in 1, 2, 4, 6 & 12 hours and intake for the day was also recorded in all groups and the results (mean±SD) were statistically analysed by Kruskal Walli’s test and p<0.05 was considered significant. All experiments were conducted adhering to strict ethical standards after getting the approval from the institutional committee.The food intake (g) and water intake (ml) were increased in the experimental groups in first hour, 4th hour, 6th hour and total intake for the day. In the high dose (p<0.01) group increase was statistically significant. The consumption for the whole day was also increased significantly (p<0.01) in the high dose group. Injection of Orexin B antagonist decreased the food and water intake significantly in the period following the infusion (p<0.05). There was an increase in feeding behaviour on infusion of Orexin B and decrease after the infusion of its antagonist into BLA. Form the results it appears that Orexin plays a role in the Basolateral amygdaloid nucleus mediated modulation of feeding behaviour. Role of orexin in ingestive behaviour is further confirmed by the action of Antagonist injection. One of the centres which mediated the orexin effects on the feeding behaviour could be BLA. Therefore Orexin B could be considered as a candidate neuroactive substance in feeding behaviuor. Further studies are being conducted in our laboratory for elucidating the role of Orexin on ingestive behaviour.