Body’s endogenous protective capabilities are challenged in different stress situations and one of challenge is adequate blood flow changes which could causes oxygen concentration large fluctuations and ischemia/reperfusion injury. Studies confirm that interorgan protection against injury could be promoted by previous short ischemic events (Przyklenk et al. 1993); even more this adaptation could be remotely promoted (Hausenloy & Yellon 2008). Remote ischemic preconditioning (RIPC) stimulus, nonlethal limb ischemia can be achieved by applying a suprasystolic blood pressure by cuff to limb. RIPC is used in cardioprotection (Hausenloy & Yellon 2008), however there exists evidence that protective linkage exists between other vascular beds (Enko et al. 2011). The mechanism, which exerts protection in a remote organ, is currently unclear (Hausenloy & Yellon 2008). The aim of study was to determine whether RIPC induces local regulatory activity changes in contralateral upper limb’s skin microcirculation. Eleven healthy young participant’s, age (24±3,yr), height (1.71±0.10,m), mass (68±8,kg) and BMI (23±2,kg/m2), without systemic or peripheral vascular diseases. Data were collected continually by single point laser Doppler imaging (moorLDI2) at the forearm’s dorsal non-glabrous skin 10 cm distally from elbow joint. After 10 min baseline was RIPC applied to contralateral limb by inflating a blood pressure cuff placed on the upper arm to 200 mmHg for 5 min and deflating the cuff for 5 min; a cycle was performed four times, followed by 10 min recovery. To evaluate local regulatory factors was used wavelet transformation, the frequency intervals of 0.0095-0.021, 0.021-0.052, and 0.052-0.145 Hz corresponding represents endothelial, sympathetic, and myogenic activity (Hodges & Del Pozzi 2014) of the microcirculatory regulation. All data are non-parametrically distributed and presented as medina (25%; 75%). To compare samples before (B) and after (A) RIPC was used Wilcoxon Signed Rank Test. Systemic circulation parameters were influenced by RIPC, statistically significant changes in mean arterial pressure (B=79.8(75.0; 88.3) vs. A=79.5(74.7; 82.7) mmHg; P=0.005) and heart rate (B=57(54;61) vs. A=55(50; 60) BPM; P<0.001) was observed, but it were not physiologically significant. Blood perfusion was not changed by RIPC (B=42(31;52) vs. A=51(37;57) PU; P=0.054) and there was not discovered significant change in endothelial activity (B=0.69(0.24;1.89) vs. A=1.02(0.42;2.20) PU2/Hz; P=0.269). However, significant change of fluctuations were observed in sympathetic (B=1.68(0.58;2.99) vs. A=2.70(0.99;4.84) PU2/Hz; P=0.035) and myogenic (B=2.42(1.00;2.92) vs. A=2.91(1.49;6.60) PU2/Hz; P=0.012) activity. RIPC induces different vascular regulatory remodulation in contralateral microvascular bed by changing sympathetic and myogenic activity of the microcirculatory regulation.