Neuropathy, dysfunction of the nerves, is a known complication of diabetes. In clinical practice neuropathy is assessed by determining patients’ ability to detect pressure using monofilaments. However, monofilaments are an insensitive assessment for neuropathy. The quick, non-invasive assessment of sural nerve activity using the novel, point of care device, NC-stat DPN-check, has been proposed to detect early evidence of neuropathy. Little is known about the variability of sural nerve activity (conductance and amplitude) in healthy individuals and individuals with type 2 diabetes but no clinical evidence of neuropathy. This study aims to examine (1) intra-participant variability of sural nerve conductance velocity and amplitude in healthy individuals and (2) whether these parameters are altered by age or diabetes. Study 1: 5 healthy individuals were recruited (age range: 20-29yrs). Sural nerve conductance and amplitude was assessed using the DPN-Check device on 5 separate occasions for each participant. Coefficient of variation (CV) was calculated (standard deviation/mean x 100) to assess intra-participant reproducibility. Study 2: 3 groups were recruited: young healthy group (n = 19, age range: 20-36yrs, 8 males); older healthy group (n =20, age range= 48-75yrs, 10 M) and a type 2 diabetes (T2DM) group with no clinical evidence of neuropathy (n = 25, age range = 56-81yrs, 21 M). Each participant underwent the assessment of sural nerve conductance and amplitude by DPN-Check. Results: Study 1: Intra-participant CVs ranged from 4.0-6.9% and 26.0-39.4% for the assessment of sural nerve conductance and amplitude, respectively. Study 2: Sural nerve conductance was lower in individuals with T2DM compared to age-matched controls (T2DM group mean (SD): 43.5 (3.4); older healthy group: 47.1 (3.4), p = 0.003, Mann-Whitney U test), but was not altered by age (young healthy group: 58.4 (3.4)). Similarly, sural nerve amplitude was significantly reduced by diabetes (p = 0.012) but not age (T2DM group: 7.1 (5.7); older healthy group: 11.7 (7.3); young healthy group: 11.0 (3.7)). The non-invasive assessment of sural nerve conductance by DPN-Check demonstrates good intra-participant reproducibility in healthy individuals. The assessment of sural nerve amplitude was more variable in these individuals. Diabetes but not age per se was associated with a reduction in sural nerve conductance and amplitude prior to the development of clinical symptoms of neuropathy.