Kinesiology tape applied to the skin with longitudinal stretch, may facilitate muscle contraction (Kase et al., 2003). However, reports on the effect of kinesiology tape on muscle contraction are equivocal. Inconsistencies within previous findings may be due to the use of measurement techniques which are not muscle specific. Tensiomyography measures electrically induced, muscle contraction of individual muscles. The aim of this study was to investigate the effect of kinesiology tape on muscle contraction, using tensiomyography. This was a block randomized, repeated measures, parallel group study. Maximal muscle contraction was elicited by incrementally increasing the simulation amplitude until no further increase in the displacement amplitude (baseline displacement measure). Contraction time was calculated from the waveform of maximum muscle contraction (baseline contraction time measure). Participants were allocated to receive either kinesiology tape using the “Y-shaped” technique (treatment group, N=31) or no tape (control group, N=31) above the dominant rectus femoris, whilst holding the leg in a position of maximum quadriceps stretch. Following treatment application, participants lay supine for 10 minutes. Subsequently, 10 consecutive measures of muscle displacement were performed 10 seconds apart, using the stimulus amplitude that elicited maximum muscle contraction at baseline. The mean displacement amplitude, and associated contraction times were calculated for the 10 measures (during treatment measure). This process was repeated after treatment removal (post treatment measure). The primary outcome measure was mean displacement amplitude. The secondary outcome measure was the associated mean contraction time. A 2 x 3 factorial repeated measures analysis of variance (ANOVA) was performed on each outcome measure, with treatment (2 levels: kinesiology tape “treatment group”, no tape “control group”) being the between-subject factors and time (3 levels: baseline, during treatment and post treatment) being the within-subject factors. Sixty-two participants (male: 29) completed this study (mean ± SD age: 23.55 ± 5.60 years; height: 171.40 ± 8.08 cm; weight: 69.16 ± 13.40 kg). There was no significant difference in mean displacement or mean contraction time between treatment groups (P=0.48, P=0.72 respectively). Time (baseline, during treatment, post treatment) did not affect mean displacement (P=0.37), but led to a significant increase in mean contraction time between baseline and during treatment (P<0.001) and between baseline and post treatment (P<0.001). There was no interaction between treatment*time (P=0.69). We provide evidence that a 10-minute application of kinesiology taping does not affect the magnitude of muscle displacement nor the duration of the associated contraction time in response to an electrically induced, involuntary contraction.