Hormonal contraceptives (HC) are the most frequently used type of birth control among young women. The use of HC may affect inflammation status (Kluft et al. 2002). In addition, using HC has been suggested to modify fat metabolism during exercise (Isacco et al. 2015). Considering the increased number of women doing high-intensity training and using HC, it is important to investigate the combined effects on inflammatory state and overall health. This pilot study examined the effects of a 10-week period of high intensity combined strength and endurance training on body composition, and inflammation marker concentrations in physically active women using hormonal contraceptives (HC, n = 9) compared to those who had never used hormonal contraceptives (NHC, n = 9). Training consisted of two strength training sessions, and two high-intensity running interval sessions per week. All the measurements were done pre and post training between days 1 – 5 of each subject’s menstrual cycle. Whole body composition was estimated by Dual X-ray Absorptiometry (LUNAR Prodigy, GE Medical Systems, Madison, USA). Automatic analyses (Encore-software, version 14.10.022) provided total body fat mass and total body lean mass. Abdominal fat was calculated manually. Fasting venous blood samples were drawn from an antecubital vein in the morning after a 12 h overnight fast. Serum was kept at -80°C until analysed for circulating high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), interleukin-1beta (IL-1b) and tumor-necrosis factor-alpha (TNF-a). The primary analytical method for assessing the change in the variables will be repeated measures analysis of variance (ANOVA). The data is expressed in mean ± SD. Total fat mass decreased significantly in NHC (-6.0 ± 6.6%, p = 0.032), where as in HC group no significant changes were observed (-6.8 ± 9.7%, p = 0.068). Circulating concentrations of hs-CRP decreased significantly in the NHC from pre to post (1.3 ± 1.2 mg/l vs. 0.7 ± 0.7 mg/l, p = 0.021), whereas no significant change was observed in HC from pre to post (1.3 ± 1.3 mg/l vs. 2.3 ± 1.9 mg/l, p = 0.40). In addition, CRP concentration was significantly higher in HC than in NHC after training (p = 0.036). Circulating TNF-α concentration significantly increased in both groups from pre to post, however no between-group differences were observed. It appears that the high-intensity exercise training can modify the inflammation status of young women. The present study shows that inflammation status in terms of hs-CRP was higher post training in HC than NHC where favorable changes in body composition were observed.