During daily life we are exposed to multiple commercially used chemicals. Although there is a large number of compounds with the potential for neurotoxicological effects on humans, there is a lack of information about the impact of these compounds on human neural development and function. Currently, the majority of neurotoxicity data reported in scientific studies is based either on in vivo animal-based models or on in vitro models with immortalized cell lines. Oftentimes, the interpretation of experimental data obtained from animal-based studies poses difficulties due to the differences between species in both neuronal anatomy and physiology. In this case, immortalized human neuronal cell lines offer a better option for in vitro screening. However, these tumor-derived cells may not display the same phenotype as primary cultures of healthy human neurons. There is an increasing need for alternative species-specific toxicology screening assays, which should have a higher reliability and effectiveness to identify and characterize the neurotoxic potential of compounds on humans. The use of human induced pluripotent stem cells (hiPSCs) and their neural derivates for assessing neurotoxicity offers many advantages, such as the potential of unlimited expansion, as well as the ability to portray not only structural, but also functional characteristics of neurons of both the CNS and PNS. In this sense, sensory neurons (SNs) derived from hiPSCs were used to establish a screening platform, which is able to analyze the effects of multiple compounds on structure and function of human SNs. For this aim, two common pyrethroid-based insecticides (deltamethrin and bioallethrin) were selected for this proof-of-concept. These insecticides are known to disrupt the kinetics of voltage-gated sodium channels in both insect and mammalian neurons by prolonging the time the channel is open. This causes neuronal hyperexcitability and is the basis for their insecticidal and toxicological mode-of-action. To distinguish between general cytotoxicity and specific neurotoxicity, the no observed effect concentrations (NOECs) of deltamethrin and bioallethrin are identified using the neutral red uptake assay. The selected test concentrations are applied on mature SNs. After exposure, both neurite morphology and spontaneous activity are assessed. Using immunostaining, neurites and cell bodies of neurons are quantified with an imaging reader. Considering the fact that many disruptions to neuronal excitability occur in the absence of morphological changes to the neurons, we proceed to assess changes in neuronal activity using the Multielectrode array (MEA) technology. Thus, we here established a human-based method, which embraces both structural and functional effects of potential neurotoxins.