Respiratory modulations can modulate cardiac autonomic tone, which is reflected in heart rate variability. All the respiratory training modules have involved volitional breaths, change in respiratory rate and/or inspiratory resistance. So the question remained that what is the effect of the act of volition has on the heart rate variability? The present study planned to find the acute impact of volitional breathing/ Conscious breathing (VB) and Breathing with minimal inspiratory resistance (IRB) (with no load on respiratory exerciser) keeping rate and depth similar to spontaneous breathing (SB). The novice volunteers (age 30 4.2 years) participated in the study (n=31 for VB; n=17 for IRB). Standard lead II electrocardiogram (ECG) and respiratory movements were recorded at rest (spontaneous breathing (SB)). After the baseline recording, subjects’ Spontaneous Breathing rate (SBR) was calculated, and then they were instructed to do either paced breathing or to breath through respiratory exerciser (POWERbreathe wellness, Southam, UK) with no load to provide minimal inspiratory resistance paced at their SBR using a Human voice-based instruction system (inspiration and expiration duration was kept at a ratio of 1:1). All recordings were done in a comfortable sitting posture in the thermoneutral lab environment. HRV analysis was done using Nevrokard BRS analysis/ver 3.2.0. Data presented in mean ± SD. Both VB and IRB were compared with respective SB by paired student t-test. HRV showed a decrease in LF (nu) (50.14 ± 21.41 vs 41.29 ± 20.52 for SB vs VB (P=0.026); 55.05 ± 14.91 vs 34.91 ± 19.36 for SB vs IRB (P=0.028)) and LF/HF ratio (1.81 ± 1.60 vs 1.13 ± 1.06 for SB vs VB (P=0.041); 1.83 ± 1.20 vs 0.75 ± 0.60 for SB vs IRB (P=0.007)) with an increase in HF (nu) (42.83 ± 23.32 vs 53.93 ± 20.98 for SB vs VB(P=0.021); 38.82 ± 16.35 vs 60.49 ± 19.93 for SB vs IRB (P=0.004)) with volitional breathing. The total power, SDNN, RMSSD, and pNN50 did not show significant change. This data suggests that, volitional breathing shifts cardiac autonomic control towards parasympathetic at the same frequency of spontaneous breathing. Abbreviations: HRV: heart rate variability VB: Volitional breathing/ Conscious breathing IRB: Breathing with minimal inspiratory resistance SB: spontaneous breathing LF (nu): Relative power of the low-frequency band (0.04-0.15 Hz) in normal units HF (nu): Relative power of the high-frequency band (0.15-0.4 Hz) in normal units LF/HF ratio: Ratio of LF-to-HF power SDNN: Standard deviation of NN intervals RMSSD: Root mean square of successive RR interval differences pNN50: Percentage of successive RR intervals that differ by more than 50 ms